Whole corticotropic hormone immunoradiometric assay kit RIA
Explore cortico-adrenal, congenital adrenal hyperplasia and glucocorticoid hypercorticism
Evaluate ACTH secretion following stimulation or suppression tests to assess hypothalamo-hypophyso-adrenal disturbances
Etiologicaly diagnosis Cushing’s syndromes: ACTH dependent or ACTH independent
Etiologically diagnosis adrenal deficiencies: primary adrenal deficiency - Addison’s disease;secondary adrenal deficiencies – panhypopituitarism, weaning off cortico-therapy
- ACCURATE AND RELIABLE ASSAY
- NO INTERFERENCE WITH PEPTIDE 1-24
Two monoclonal anti-ACTH molecule antibodies are used for the sandwich. The first one, specific to the N-terminal part of ACTH, is coated on the ELSA solid phase, while the second one, specific to the C-terminal part of ACTH, radiolabelled with iodine-125, is used as the tracer.
One-step assay procedure:18-22h at RT.
Working range: 2 – 2000 pg/mL
Sample: EDTA plasma – 200 µL
Analytical sensitivity: 2 pg/mL
|Concentration range pg/mL||Number of patients||%|
|0 - 10||0||0|
|80 - 90||2||2|