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Viral double-stranded RNA detection kit HTRF®

The dsRNA kit isdesigned to detect double-stranded RNA, a trait shared by all viruses and a sign of host-cell infection, in cell lysates.
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  • Ready-to-use Ready-to-use
  • Ease-of-use Ease-of-use
  • Cell-based Cell-based
The dsRNA kit isdesigned to detect double-stranded RNA, a trait shared by all viruses and a sign of host-cell infection, in cell lysates.
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Overview

Double-stranded RNA (dsRNA) generated during genome replication is a commonality to many viruses. Traditional methods for detecting virus infection have relied on titering virus particles (plaque assay). These methods have proven lengthy, often taking place over several days. This assay detects the presence of dsRNA in cell lysates, a byproduct of viral replication activity, making it possible to obtain same-day results.

Benefits

  • ALTERNATIVE TO THE PLAQUE ASSAY
  • SAME-DAY RESULTS
  • COMPATIBLE WITH MANY STRAINS

Assay principle

dsRNA is detected in a sandwich assay format using two antibodies, one labelled with Europium Cryptate (donor) and the second with d2 (acceptor). The detection principle is based on HTRF technology. When the dyes are in close proximity, the excitation of the donor with a light source (laser or flash lamp) triggers a Fluorescence Resonance Energy Transfer (FRET) towards the acceptor, which in turn fluoresces at a specific wavelength (665 nm). The two antibodies bind to the dsRNA present in the sample, thus generating FRET. Signal intensity is proportional to dsRNA concentration.
dsRNA detection kit principle

Assay Protocol

The assay protocol, using a 384-well small volume white plate or a Cisbio low volume 96 well plate (20 µL final), is described on the right. 10 µL of cell lysates or control are dispensed directly into the detection plate for detection by HTRF reagents. The antibodies labelled with HTRF donor and acceptor can be pre-mixed and added in a single dispensing step to further streamline the assay procedure. The assay can be run in 96- to 384-well plates by simply resizing each addition volume proportionally.
dsRNA detection kit protocol

Detection of synthetic dsRNA

The signal was obtained on a range of concentrations of a synthetic dsRNA template diluted in the kit's lysis buffer. The synthetic dsRNA is used to mimic the dsRNA produced upon viral infection and subsequent genome replication.
Viral dsRNA detection kit's standard curve

Product Insert Viral dsRNA Kit / 64RNAPEG-64RNAPEH

64RNAPEG-64RNAPEH - Product Insert

HTRF Product Catalog 2020 July update

All your HTRF assays in one document! - Catalog

A guide to Homogeneous Time Resolved Fluorescence

General principles of HTRF - Guides

How HTRF compares to Western Blot and ELISA

Get the brochure about technology comparison. - Brochures

Innovative solutions in Virology research

Kits and reagents for virology research - Flyers

Plate Reader Requirement

Choosing the right microplate reader ensures you’ll get an optimal readout. Discover our high performance reader, or verify if your lab equipment is going to be compatible with this detection technology.

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