We are delighted to announce the launch of new HTRF products
The HTRF Utrophin kit is designed for the rapid detection of Utrophin in cell lysates.
- All inclusive kit
- Highly specific
- Faster and more convenient than ELISA
|Utrophin is an autosomally-encoded 376-kDa cytoskeletal protein that is similar in structure and function to dystrophin. It is a ubiquitously expressed protein that plays a role in anchoring the cytoskeleton to the plasma membrane. Utrophin is highly expressed in developing muscle, and is enriched at the neuromuscular junction of mature muscle. Duchenne's muscular dystrophy patients lack dystrophin, and utrophin is consequently up-regulated and redistributed to locations normally occupied by dystrophin. Cisbio's Utrophin kit is designed for its rapid detection in cell lysates.|
- Cost-effective alternative to ELISA
- Measures Mouse and Human Utrophin
- Validated on HeLa and C2C12 cells
The Utrophin assay features a two-plate assay protocol, where cells are first plated, stimulated, and lysed in the same culture plate. Lysates are then transferred to the assay plate for the detection of utrophin. This protocol enables the cells' viability and confluence to be monitored. The antibodies labelled with HTRF fluorophores may be pre-mixed and added in a single dispensing step to further streamline the assay procedure. The assay detection can be run in 96- to 384-well plates by simply resizing each addition volume proportionally.
Human HeLa cells were plated under 100 µl in 96-well plates at different cell densities (100K, 50K, 25K, 12.5K, 6.25K, and 3.1K cells/well) in complete culture medium, and incubated at 37°C, 5% CO2. The day after, medium was removed and cells were then lysed with 50 µL of lysis buffer #3 for 30 minutes at room temperature under gentle shaking. Next, 16 µL of lysate were transferred into a low volume white microplate before the addition of 4 µL of the premixed HTRF Utrophin detection reagents. The HTRF signal was recorded after ovenight incubation.
Mouse C2C12 cells were plated under 100 µl in a 96-well plates at different cell densities (200K, 100K, 50K, 25K, 12.5K, and 6.25K cells/well) in complete culture medium and incubated at 37°C, 5% CO2. The day after, medium was removed and cells were then lysed with 50 µL of lysis buffer #3 for 30 minutes at room temperature under gentle shaking Next, 16 µL of lysate were transferred into a low volume white microplate before the addition of 4 µL of the premixed HTRF Utrophin detection reagents. The HTRF signal was recorded after overnight incubation.
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