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Ultra sensitive Human total estradiol-17beta-E2 RIA RIA

The ESTR-US-CT kit is a radioimmunoassay of human total estradiol-17ß and constitutes an outstanding tool for investigations into fertility and oncology disorders.
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The ESTR-US-CT kit is a radioimmunoassay of human total estradiol-17ß and constitutes an outstanding tool for investigations into fertility and oncology disorders.
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In stock

Overview

Assess puberty in young girls with clinical signs of an estrogen hypersecretion

Assess menopause

Identify estrogen secreting tumors

Monitor fertility

Evaluate hyperestrogenism in men

Benefits

  • EXCELLENT SENSITIVITY
  • VERY LOW CROSS-REACTIVITY WITH ESTRONE (0.97%)

Assay principle: competition

The principle of the assay is based on a two-step competition. First, the samples are incubated in the coated tubes with anti-estradiol polyclonal antibodies adsorbed to the wall. A 125I-estradiol tracer is then added to saturate the remaining free antibody sites. Any unbound tracer is then removed.

Assay procedure: 1h at 37°C + 1 h at 37°C.

Features

Working range: 5 – 2000 pmol/L (standards are prepared gravimetrically)

Sample: serum or heparin, EDTA plasma – 200 µL

Analytical sensitivity: 5 pmol/L

Normal values

Phases Days from the LH peak Healthy normally menstruating women
n
Mean
17ß-E2 pmol/L
Range
17ß-E2 pmol/L
Follicular phase - 8 10 150 105 - 217
-4 15 343 207 - 1000
Mid-cycle -1 24 709 416 - 1399
Luteal phase 9 17 399 165 – 788
Group of women Age (y) n Mean
17ß-E2 pmol/L
Range
17ß-E2 pmol/L
Postmenopausal without hormone replacement therapy 47 – 66 42 26 11 - 50
Pre-pubertal girls 3 - 5 42 17 ND – 36
Men n = 117 17ß-E2 pmol/L
Mean 117
Reference interval 34 – 226
Confidence intervals 0.90
Lower reference limit 23 - 51
Upper reference limit 191 - 266

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Annual availability Schedule 2020

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Product Insert (ENG) ESTR-US-CT / ESTR-US-CT

ESTR-US-CT Op.Mod.021 - Product Insert

Product Insert (FRA) ESTR-US-CT / ESTR-US-CT

ESTR-US-CT Op.Mod.021 - Product Insert

Certificat ESTR-US-CT / ESTR-US-CT

ESTR-US-CT - Certificat

Quality Control Report ESTR-US-CT / ESTR-US-CT

ESTR-US-CT Batch 97A(1h+1h) - Quality Control Report

Quality Control Report ESTR-US-CT / ESTR-US-CT

ESTR-US-CT Batch 99A(1h+1h) - Quality Control Report