Two screen islet cell autoantibody (GADAb & IA-2Ab) ELISA kit ELISA

The 2 Screen ELISA test measures levels of glutamic acid decarboxylase and IA-2 autoantibodies in a single serum sample to identify type I diabetes mellitus.
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The 2 Screen ELISA test measures levels of glutamic acid decarboxylase and IA-2 autoantibodies in a single serum sample to identify type I diabetes mellitus.
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Overview

Use as serological markers of type 1 diabetes mellitus (insulin-dependent diabetes mellitus - IDDM)

Benefits

  • ACCURATE & RELIABLE ENZYMATIC ASSAY

Assay principle: sandwich

GAD65 and IA-2 molecules are coated onto the ELISA plate wells. GAD65-biotin and IA-2-biotin complexes constitute the liquid phase. GAD-Ab and IA-2-Ab from the sample bind to the GAD65 and to IA-2 immobilized onto the well and interact with GAD65-biotin and IA-2 biotin via a GAD-Ab and IA-2-Ab bridges. Addition of streptavidin peroxidase detects the bound GAD65-biotin and IA-2 biotin.

The unbound streptavidin peroxidase in excess is washed off before the addition of the peroxidase substrate TMB. Assay procedure: 16-20h at 2-8°C + 1h at RT / shaking + 20 min at RT / shaking + 20 min at RT in the dark.

Features

Working range: 4 - 450 U/mL (NIBSC 97/550)

Sample: serum - 50 µL Analytical sensitivity: 0.17 U/mL

Normal values

Cut-off2Screen U/mL
Negative
Positive= 4

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