Total FGFR2 cellular kit HTRF®

The total FGFR2 kit monitors the cellular FGFR2 expression level and can be used as a normalization assay for the phospho-FGFR kit.
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  • Ease-of-use Ease-of-use
  • Highly specific Highly specific
The total FGFR2 kit monitors the cellular FGFR2 expression level and can be used as a normalization assay for the phospho-FGFR kit.
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The Total FGFR2 cellular assay monitors total FGFR and is used as a normalization assay with the phospho-FGFR kit. Mutations in FGFR2 have been associated with cancer (breast, melanoma) and abnormal bone development (cranosynostosis syndromes).



Total-FGFR2 assay principle

The Total-FGFR2 assay quantifies the expression level of FGFR2 in a cell lysate. Contrary to Western Blot, the assay is entirely plate-based and does not require gels, electrophoresis or transfer. The Total-FGFR2 assay uses two labeled antibodies: one coupled to a donor fluorophore, the other to an acceptor. Both antibodies are highly specific for a distinct epitope on the protein. In presence of FGFR2 in a cell extract, the addition of these conjugates brings the donor fluorophore into close proximity with the acceptor and thereby generates a FRET signal. Its intensity is directly proportional to the concentration of the protein present in the sample, and provides a means of assessing the protein’s expression under a no-wash assay format.
Total FGFR2 assay principle

Total-FGFR2 2-plate assay protocol

The 2 plate protocol involves culturing cells in a 96-well plate before lysis then transferring lysates to a 384-well low volume detection plate before adding Total FGFR2 HTRF detection reagents. This protocol enables the cells' viability and confluence to be monitored.
Total FGFR2 2-plate assay protocol

Total-FGFR2 1-plate assay protocol

Detection of total FGFR2 with HTRF reagents can be performed in a single plate used for culturing, stimulation and lysis. No washing steps are required. This HTS designed protocol enables miniaturization while maintaining robust HTRF quality.
Total FGFR2 1-plate assay protocol

Detection phospho and total FGFR2 on Kato-III cells

Kato-III cells were plated in 96-well plate and incubated overnight. Cells were treated with increasing concentration of FIIN-1 hydrochloride compound for 6h at 37 °C - 5% CO2. After stimulation, cells were washed and lysed with lysis buffer#2 1X for 30 min at RT under gentle shaking. 16 µL of lysate were transferred into a 384-well sv white microplate and 4 µL of the HTRF phospho FGFR2 or total FGFR2 detection reagents were added. The HTRF signal was recorded after a overnight incubation at room temperature.
Detection phospho and total FGFR2 on Kato-III cells

Simplified pathway dissection with HTRF phospho-assays and CyBi-felix liquid handling

Analyse of PI3K/AKT/mTor translational control pathway - Application Notes

Open R&D: Sanofi Access Platform

In collaboration with Sanofi - Scientific Presentations

Cisbio lysis buffer compatibility

Cell Signaling: Biomarkers, Phospho- & total-protein Assays - Flyers

HTRF cellular phospho-protein assays

Physiologically relevant results fo fast flowing research - Flyers

Species compatibility

Cell Signaling: Biomarkers, Phospho- & total-protein assays - Flyers

Universal HTRF® phospho-protein platform: from 2D, 3D, primary cells to patient derived tumor cells

Analysis of a large panel of diverse biological samples and cellular models - Posters

HTRF phospho assays reveal subtle drug induced effects in tumor-xenografts

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HTRF cell-based phospho-protein data normalization

Valuable guidelines for efficiently analyzing and interpreting results - Application Notes

HTRF phospho-total lysis buffer: a universal alternative to RIPA lysis buffers

Increased flexibility of phospho-assays - Application Notes

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Insider Tips for successful sample treatment - Technical Notes

HTRF Alpha-tubulin Housekeeping kit

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Optimize your HTRF cell signaling assays on tissues

HTRF and WB compatible guidelines - Technical Notes

Key guidelines to successful cell signaling experiments

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HTRF phospho-assays reveal subtle drug-induced effects

Detailed protocol and direct comparison with WB - Posters

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What to expect at the bench - Videos

Unleash the potential of your phosphorylation research with HTRF

Unmatched ease of use, sensitivity and specificity assays - Videos

Product Insert FGFR2 total Kit / 63ADK057PEG-63ADK057PEH

63ADK057PEG-63ADK057PEH - Product Insert

HTRF Product Catalog 2020 July update

All your HTRF assays in one document! - Catalog

A guide to Homogeneous Time Resolved Fluorescence

General principles of HTRF - Guides

How HTRF compares to Western Blot and ELISA

Get the brochure about technology comparison. - Brochures

HTRF® cell signaling platform combined with iCell® Hepatocytes

A solution for phospho-protein analysis in metabolic disorders - Posters

Unleash the potential of your phosphorylation research with HTRF

A fun video introducing you to phosphorylation assays with HTRF - Videos

How to run a cell based phospho HTRF assay

3' video to set up your Phospho assay - Videos

Plate Reader Requirement

Choosing the right microplate reader ensures you’ll get an optimal readout. Discover our high performance reader, or verify if your lab equipment is going to be compatible with this detection technology.

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