Thyroid Peroxidase Autoantibody (TPO-AB) ELISA kit ELISA

The PE/96/2 kit is an accurate enzymatic assay to measure the thyroid peroxidase autoantibodies (TPO-AB) in human serum to diagnose autoimmune thyroid disorders.
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The PE/96/2 kit is an accurate enzymatic assay to measure the thyroid peroxidase autoantibodies (TPO-AB) in human serum to diagnose autoimmune thyroid disorders.
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Overview

Diagnosis autoimmune thyroid diseases

Identify autoimmune destruction of the thyroid associated with the formation of autoantibodies to thyroid peroxidase

Benefits

  • ACCURATE AND RELIABLE ENZYMATIC ASSAY

Assay principle: sandwich

TPO molecules are coated onto the ELISA plate wells. A Protein A-Alkaline Phosphatase conjugate constitutes the liquid phase, which binds to the TPOAb from the sample bound to the immobilized TPO. The addition of p-nitrophenyl phosphate (pNPP) enables quantification of the Protein a-Alkaline phosphatase conjugate bound to the plate.

Assay procedure: 15 min at RT / shaking + 15 min at RT / shaking + 15 min at RT in the dark.

Features

Working range: 10 – 5000 U/mL (WHO 66/387)

Sample: serum – 50 µL

Analytical sensitivity: 1.05 U/mL

Normal values

Cut-offTPOAb U/mL
Negative<10
Positive>= 10

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