Estradiol kit
Estradiol quantification from cells, serum and enzymatic assays
Testosterone kit HTRF®
The Testosterone kit is designed for the fast quantification of the steroid hormone in cell-based and enzymatic formats.
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No-wash
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Low sample consumption
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High sensitivity
Overview
Testosterone is a steroid hormone synthesized from 17a-hydroxy progesterone by the enzymes 17,20-lyase and 17ß-hydroxysteroid dehydrogenase in the gonads of both males and females, although small amounts are also secreted by the adrenal glands. It is the principal male sex hormone and an anabolic steroid. It plays an important role in the stimulation and the development of masculine characteristics by its fixation of androgen receptors.
Benefits
- 17ß-HYDROXYSTEROID DESHYDROGENASE MONITORING
Assay principle
The testosterone assay is based on the competition principle, where native testosterone produced by cells and XL665-labelled testosterone compete for binding to a monoclonal anti-testosterone antibody labelled with Europium Cryptate.
Assay Protocol
The testosterone assay features a streamlined protocol with only two incubation steps: - Cell stimulation by target compounds - Testosterone detection using HTRF reagents
This protocol requres a single one-hour incubation period following cell stimulation.
Key features
| Detection limit: | 0.1 nM |
|---|---|
| Dynamic range: | 0.1 to 10 nM |
| EC50 | 0.84 nM |
| S/B | 13 |
| Specificity: | low cross-reactivity with 4-Androstene-3,17-Dione 1.3% |
Product Insert Testosterone Kit / 64TESPEG-64TESPEH
64TESPEG-64TESPEH - Product Insert
HTRF Product Catalog 2020 July update
All your HTRF assays in one document! - Catalog
A guide to Homogeneous Time Resolved Fluorescence
General principles of HTRF - Guides
How HTRF compares to Western Blot and ELISA
Get the brochure about technology comparison. - Brochures
Plate Reader Requirement
Choosing the right microplate reader ensures you’ll get an optimal readout. Discover our high performance reader, or verify if your lab equipment is going to be compatible with this detection technology.
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