Identification of cytokine-signaling inhibitors contributes a great deal...
Streptavidin-XL665 HTRF®
XL665-labeled Streptavidin for capturing biotinylated proteins, peptides, antibodies, small molecules, nucleic acids....
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No-wash
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Ease-of-use
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High affinity
Overview
Streptavidin has been labeled with XL665. Biotin binds to Streptavidin with high affinity (Ka=1015M-1). The binding is rapid and stable, making it an ideal choice for use in a variety of assays such as enzyme assays, protein-protein binding assays and molecular biology assays.
Benefits
- LARGE COMPLEXES DETECTION
- BATCH-TO-BATCH REPRODUCIBILITY
- LOW TO HIGH KD DETECTION
Assay principle
Assay protocol
The example on the right describes the protocol using a 20 µL final assay volume for detecting an interaction between a biotinylated-tagged partner A and a non-tagged partner B*.
Dispense the 2 partners (10 µL), incubate, add Streptavidin-XL (5 µL) and anti-partner B labeled with donor (5 µL), incubate and read.
*partner B can also be tagged, Fc fused or directly labeled. In these cases use for the detection, the corresponding HTRF reagent (anti-Tag, anti species, protA, Streptavidin), labeled with donor.
Build HTRF interaction assays for your specific application
How do the number of tests relate to active moiety?
Recommended quantities of Cryptate and XL665 conjugates
Exploiting HTRF for novel drug classes: Stabilizing 14-3-3 protein-protein interactions
In collaboration with Lead Discovery Center GmbH - Scientific Presentations
Experience the freedom of building your own assay
HTRF kinase activity toolbox reagents - Flyers
HTRF protein-protein interaction reagents
Benefit from unlimited flexibility for your assay development - Flyers
Addressing the interactome with protein-protein assays
Cover all PPIs with one approach - Brochures
HTRF -A Beneficial Tool for Lead Optimization of Biotherapeutics
In collaboration with Boehringer Ingelheim - Posters
Setting-up HTRF technology for G9a & DNMT1 High-Throughput Screening
In collaboration with Universidad de Navarra - Posters
The ultimate guide to PPI assays with HTRF
Complete guide - Guides
Guidelines for optimizing protein-protein interaction assays using HTRF PPI reagents
Every interaction is within range - Technical Notes
Product Insert Streptavidin-XL / 610SAXLF-610SAXLA-610SAXLB
610SAXLF-610SAXLA-610SAXLB - Product Insert
HTRF PPI your dream assay served on a plate
Sandwiches aren't just for eating - Infographics
Best practices for pharmacological characterization of PPI inhibitors
Easy pharmacological characterization of PPI modulators. - Technical Notes
HTRF assays handle low- to high affinity protein-protein interactions
Deciphering low- and high affinity interactions - Application Notes
Nuclear receptor ligand identification with HTRF
Monitoring nuclear receptor binding with HTRF assays - Application Notes
HTRF addresses large protein-protein interaction complexes
Challenge large complexes with HTRF assays - Application Notes
A brief history of Protein-Protein Interactions
How well do you know PPI? - Infographics
HTRF Product Catalog 2020 July update
All your HTRF assays in one document! - Catalog
A guide to Homogeneous Time Resolved Fluorescence
General principles of HTRF - Guides
How HTRF compares to Western Blot and ELISA
Get the brochure about technology comparison. - Brochures
Virology research solutions using HTRF Protein-Protein Interaction assays
See how peer researchers challenge the viral life cycle with PPI assays - Application Notes
Advance your CAR-T cell research - Flyers
Safety Data Sheet Streptavidin-XL / 610SAXAC
610SAXAC - Safety Data Sheet
Safety Data Sheet Streptavidin-XL / 610SAXLA
610SAXLA - Safety Data Sheet
Safety Data Sheet (DEU) Streptavidin-XL / 610SAXLA
610SAXLA - Safety Data Sheet
Safety Data Sheet (ELL) Streptavidin-XL / 610SAXLA
610SAXLA - Safety Data Sheet
Safety Data Sheet (FRA-FR) Streptavidin-XL / 610SAXLA
610SAXLA - Safety Data Sheet
Safety Data Sheet (ITA) Streptavidin-XL / 610SAXLA
610SAXLA - Safety Data Sheet
Safety Data Sheet (SPA) Streptavidin-XL / 610SAXLA
610SAXLA - Safety Data Sheet
Safety Data Sheet (ENG-GB) Streptavidin-XL / 610SAXLA
610SAXLA - Safety Data Sheet
Safety Data Sheet (ENG-US) Streptavidin-XL / 610SAXLA
610SAXLA - Safety Data Sheet
Safety Data Sheet Streptavidin-XL / 610SAXLB
610SAXLB - Safety Data Sheet
Safety Data Sheet (DEU) Streptavidin-XL / 610SAXLB
610SAXLB - Safety Data Sheet
Safety Data Sheet (ELL) Streptavidin-XL / 610SAXLB
610SAXLB - Safety Data Sheet
Safety Data Sheet (FRA-FR) Streptavidin-XL / 610SAXLB
610SAXLB - Safety Data Sheet
Safety Data Sheet (ITA) Streptavidin-XL / 610SAXLB
610SAXLB - Safety Data Sheet
Safety Data Sheet (SPA) Streptavidin-XL / 610SAXLB
610SAXLB - Safety Data Sheet
Safety Data Sheet (ENG-GB) Streptavidin-XL / 610SAXLB
610SAXLB - Safety Data Sheet
Safety Data Sheet (ENG-US) Streptavidin-XL / 610SAXLB
610SAXLB - Safety Data Sheet
Safety Data Sheet (DEU) Streptavidin-XL / 610SAXLF
610SAXLF - Safety Data Sheet
Safety Data Sheet (ELL) Streptavidin-XL / 610SAXLF
610SAXLF - Safety Data Sheet
Safety Data Sheet (FRA-FR) Streptavidin-XL / 610SAXLF
610SAXLF - Safety Data Sheet
Safety Data Sheet (ITA) Streptavidin-XL / 610SAXLF
610SAXLF - Safety Data Sheet
Safety Data Sheet (SPA) Streptavidin-XL / 610SAXLF
610SAXLF - Safety Data Sheet
Safety Data Sheet (ENG-GB) Streptavidin-XL / 610SAXLF
610SAXLF - Safety Data Sheet
Safety Data Sheet (ENG-US) Streptavidin-XL / 610SAXLF
610SAXLF - Safety Data Sheet
Safety Data Sheet Streptavidin-XL / 610SAXLG
610SAXLG - Safety Data Sheet
Safety Data Sheet (DEU) Streptavidin-XL / 610SAXLG
610SAXLG - Safety Data Sheet
Safety Data Sheet (ELL) Streptavidin-XL / 610SAXLG
610SAXLG - Safety Data Sheet
Safety Data Sheet (FRA-FR) Streptavidin-XL / 610SAXLG
610SAXLG - Safety Data Sheet
Safety Data Sheet (ITA) Streptavidin-XL / 610SAXLG
610SAXLG - Safety Data Sheet
Safety Data Sheet (SPA) Streptavidin-XL / 610SAXLG
610SAXLG - Safety Data Sheet
Safety Data Sheet (ENG-GB) Streptavidin-XL / 610SAXLG
610SAXLG - Safety Data Sheet
Safety Data Sheet (ENG-US) Streptavidin-XL / 610SAXLG
610SAXLG - Safety Data Sheet
Plate Reader Requirement
Choosing the right microplate reader ensures you’ll get an optimal readout. Discover our high performance reader, or verify if your lab equipment is going to be compatible with this detection technology.
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