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Somatomedin C (IGF-I) immunoradiometric assay kit

Somatomedin C (IGF-I) immunoradiometric assay kit RIA

The IGF1-RIACT kit is an immunoradiometric assay for somatomedin C (IGF-I) to explore and monitor growth disorders in children and adults.

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The IGF1-RIACT kit is an immunoradiometric assay for somatomedin C (IGF-I) to explore and monitor growth disorders in children and adults.

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Overview

Diagnosis and monitor treatment for growth-related disorders in children

Assess adult hGH deficiency

Monitor acromegaly treatment

Assess nutritional state

Benefits

NO INTERFERENCE WITH BINDING PROTEINS
NO EXTRACTION NECESSARY

Assay principle: sandwich

Two monoclonal antibodies directed against two different antigenic sites of the IGF-I molecule are used for the sandwich. The first one is adsorbed onto the tube walls. The second one, radiolabeled with iodine-125, is used as the tracer. A predilution of the samples (1/31) with an acidic solution is required to strip the carrier protein (BP). This carrier protein is then saturated by IGF-II. The use of IGF-II molecules in the assay avoids any reassociation between IGF-I and the carrier protein (IGF-BP).

Assay procedure: 2h at RT / shaking + 2h at RT / shaking.

Features

Working range: 1 – 1000 ng/mL (IRS 87/518)

Sample: serum or plasma – 25 µL - predilution required (1/31) Analytical sensitivity: 1 ng/mL

Normal values for adults

Population	Age (years)	18 - 20		21 - 23		24 - 26		27 - 29		30 - 39		40 - 49		50 - 59		60 - 69		70 - 89
Population	Age (years)	n	IGF-I ng/mL	n	IGF-I ng/mL	n	IGF-I ng/mL	n	IGF-I ng/mL	n	IGF-I ng/mL	n	IGF-I ng/mL	n	IGF-I ng/mL	n	IGF-I ng/mL	n	IGF-I ng/mL
Men		57		63		54		50		56		51		54		49		34
	Median		317		279		251		233		209		178		159		149		132
	2.5th percentile		197		173		155		143		127		107		94		87		75
	97.5th percentile		486		430		389		363		329		286		262		250		231
Women		42		55		45		48		47		50		54		48		50
	Median		339		310		285		264		225		184		153		131		122
	2.5th percentile		169		159		150		142		126		107		90		76		67
	97.5th percentile		517		476		440		410		356		297		247		209		189

For Children

Population		Normal size children
Pubertal stage	Age (years)	n	5th percentile	Median	95th percentile
Pubertal stage	Age (years)	n	IGF-I ng/mL	IGF-I ng/mL	IGF-I ng/mL
Tanner I	0 – 2	42	50	86	143
Tanner I	2 – 4	24	51	121	218
Tanner I	4 – 8	51	106	163	250
Tanner I	8 – 10	38	126	194	261
Tanner I	> 10	13	140	242	496
Tanner II		20	198	294	551
Tanner III		22	238	430	672
Tanner IV		20	312	470	870
Tanner V		11	302	451	774

Population		Constitutionally small size children
Pubertal stage	Age (years)	n	5th percentile	Median	95th percentile
Pubertal stage	Age (years)	n	IGF-I ng/mL	IGF-I ng/mL	IGF-I ng/mL
Tanner I	< 8	30	34	69	145
Tanner I	8 – 10	19	46	91	207
Tanner I	> 10	49	85	151	236
Tanner II		18	130	166	361
Tanner III		13	147	277	462
Tanner IV		9	198	325	442