Sex Hormone Binding Globulin immunoradiometric assay kit RIA

The SHBG-RIACT kit is an immunoradiometric assay principle for SHBG in case of polycytic ovary syndrome and male hypogonadism

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The SHBG-RIACT kit is an immunoradiometric assay principle for SHBG in case of polycytic ovary syndrome and male hypogonadism

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Overview

Calculate the free androgen index: FTI = total testosterone / SHBG x 100

Assess hyperandrogenism etiology (hirsutism, virilism)

Evaluate polycystic ovary syndrome

Evaluate male hypogonadism

Benefits

LOW DETECTION LIMIT

Assay principle: sandwich

Two monoclonal anti-SHBG antibodies are used for the sandwich. The first one is adsorbed onto the tube walls. The second one, radiolabeled with iodine-125, is used as the tracer.

Assay procedure: 30 min at RT / shaking + 30 min at RT / shaking.

Features

Working range: 0.5 – 200 nmol/L

Sample: serum or plasma – 10 µL

Analytical sensitivity: 0.5 nmol/L

Normal values

Sites	Men			Menstrual women
Sites	n	Mean ± SD (nmol/L)	Range (nmol/L)	n	Mean ± SD (nmol/L)	Range (nmol/L)
Site 1	23	29.7 ± 12.2	9 54	23	47.9 ± 12.9	30 69
Site 2	20	26.1 ± 10.6	12 46	15	62.1 ± 14.3	35 - 87
Site 3	30	27.5 ± 7.7	15 - 43	30	50.5 ± 16.6	16 - 83