Serotonin 5HT1AR fluorescent probe
Tag-lite Serotonin 5HT1A receptor fluorescent probe
The 5-HT1A receptor is a GPCR widely distributed in regions that receive serotonergic input, including the frontal cortex, septum, amygdala, hippocampus, and hypothalamus. The serotonin 1A receptor (5-HT1A) plays a role in the genesis of several major depressive disorders.
Cells expressing the 5HT1A receptor are provided pre-labeled with Terbium, and can be used to conduct receptor binding studies on the aforementioned receptor.
Running your 5HT1A receptor binding assay using Tag-lite is as easy as it can gets. Simply dispense 10 µL of labeled cells into each well, followed by 5 µL of labeled ligand and 5 µL of the compound you wish to test. Like all HTRF assays, Tag-lite assays do not require any washing steps. A diagram of the procedure to be followed is given on the right.
Examples of data obtained using Serotonin 5HT1A labeled cells and their matching fluorescent ligand (L0029RED). WAY-100135 was used as reference ligand. Results may vary from one HTRF® compatible reader to another.
In collaboration with Boehringer Ingelheim - Scientific Presentations
In collaboration with Bayer - Scientific Presentations
Challenge the limits of binding kinetics studies - Application Notes
Featuring a panel of experts - Videos
The gold standard technology for receptor binding studies - Videos
Get the brochure about technology comparison. - Brochures
Available On-demand - Videos
How to revolutionize your kinetic binding demonstration with HTRF kinase binding platform assays - Application Notes
Choosing the right microplate reader ensures you’ll get an optimal readout. Discover our high performance reader, or verify if your lab equipment is going to be compatible with this detection technology.Let's find your reader
SNAP tag 5HT1A 5-Hydroxytryptamine 1AR cloned plasmid
Plasmid cloned with a SNAP tag 5HT1A 5-Hydroxytryptamine 1A receptor