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SARS-Cov2 Spike S1 protein detection kit HTRF®

This HTRF kit is designed to detect SARS-CoV-2 Spike S1 proteins in cell lysates or cell supernatant, as a marker of viral infection.

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  • No-wash No-wash
  • Low sample consumption Low sample consumption
  • High sensitivity High sensitivity

This HTRF kit is designed to detect SARS-CoV-2 Spike S1 proteins in cell lysates or cell supernatant, as a marker of viral infection.

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Overview

The SARS-CoV-2 Spike S1 protein is one of two part of the virus Spike protein (S). The S1 subunit is responsible for the the virus interaction with host cells' transmembrane Angiotensin-converting enzyme 2 receptors (ACE2), while the S2 subunit is capable of conformational changes that allow the fusion of the viral particle envelope and host cell membrane upon S1/ACE2 binding. As such, the S1 protein is closely investigated for its role in viral infection and is reliable marker of infection as well as a potential vaccine immunogen target.

SARS-CoV-2 particles are detected in cell lysates using a sandwich immunoassay involving an anti-S1 monoclonal antibody, labelled with terbium-Cryptate or d2, ensuring assay quality, reproducibility, and signal quality. The specific HTRF signal generated is proportional to the amount of S1 proteins.
Principle of the SARS-CoV-2 S1 protein assay
The assay protocol, using a 384-well small volume plate or a low volume 96 well plate (20 µL final), is described on the right. 16 µL of cell lysates or control are dispensed directly into the detection plate for detection by HTRF reagents. The antibodies labelled with HTRF donor and acceptor can be pre-mixed and added in a single dispensing step to further streamline the assay procedure (4 µL), or separately (2 µL).
Protocol of the SARS-CoV-2 S1 protein assay

Detection of recombinant SARS-CoV-2 S1 protein

16 µL of recombinant protein in lysis buffer #5 were dispensed to a low volume white microplate before the addition of 4 µL of the premixed HTRF anti SARS-CoV2 capsid detection reagents. The HTRF signal was recorded after ON incubation.
SARS-CoV-2 nucleocapsid standard curve

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Plate Reader Requirement

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