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SARS-Cov2 nucleocapsid detection kit HTRF®

This HTRF kit is designed to detect SARS-CoV-2 nucleocapsid proteins in cell lysates or cell supernatant, as a marker of viral infection.

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This HTRF kit is designed to detect SARS-CoV-2 nucleocapsid proteins in cell lysates or cell supernatant, as a marker of viral infection.

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Overview

The SARS-CoV-2 N protein is the basal monomeric constituent of the coronavirus oligomeric nucleocapsid. This protein is major immunogen predominantly expressed at early stages of viral infection and present from day one in infected patients’ serum. As such, SARS-CoV-2 N protein is a reliable marker for viral infection and a possible immunogen candidate for the development of a vaccine.

SARS-CoV-2 particles are detected in cell lysates using a sandwich immunoassay involving an anti-nucleocapsid monoclonal antibody, labelled with terbium-Cryptate or d2, ensuring assay quality, reproducibility, and signal quality. The specific HTRF signal generated is proportional to the amount of nucleocapsid proteins.
Principle of the SARS-CoV-2 nucleocapsid assay
The assay protocol, using a 384-well small volume plate or a low volume 96 well plate (20 µL final), is described on the right. 16 µL of cell lysates or control are dispensed directly into the detection plate for detection by HTRF reagents. The antibodies labelled with HTRF donor and acceptor can be pre-mixed and added in a single dispensing step to further streamline the assay procedure (4 µL), or separately (2 µL).
Protocol of the SARS-CoV-2 nucleocapsid assay

Detection of recombinant SARS-CoV-2 nucleocapsid

16 µL of recombinant protein in lysis buffer #5 were dispensed to a low volume white microplate before the addition of 4 µL of the premixed HTRF anti SARS-CoV2 capsid detection reagents. The HTRF signal was recorded after ON incubation at RT.
SARS-CoV-2 nucleocapsid standard curve

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Plate Reader Requirement

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