A marker for monitoring liver fibrosis in MTX-treated patients
Procollagen-III-peptide (PIIINP) ELISA kit (CE) ELISA
Detect the transformation of functioning liver tissue into connective tissue (in alcoholic or virus induced forms of liver fibrosis, …)
Detect and monitor the course of liver disorders (fibrosis and cirrhosis), in particular psoriatic patients treated by MTX and non-alcoholic fatty liver damage (NAFLD)
Reduce the number of biopsies for improved patient comfort and cost savings.
Detect lung fibrosis, chronic polyarthritis, collagenosis, myocardial infection, acromegaly and multiple traumas
Monitor disease progression
- ONE-STEP ELISA ASSAY AT R.T.
- AUTOMATION FRIENDLY
A monoclonal antibody, immobilized on the microplate, captures the PIIINP proteins contained in the calibrators and samples. The bound proteins are then recognized by a second monoclonal antibody conjugated to HRP (Horse-Radish peroxidase). The unbound reagents are eliminated by washing. Then, the colorimetric reaction is started by the addition of the HRP substrate TMB and stopped by addition of an acid solution. The optical density (OD) of each well is read at 450 nm. A predilution at 1/11 is required for controls and samples.
Assay procedure: 3h at RT / shaking 700rpm + 15 min at RT.
Working range: 0.036 – 30 µg/L
Sample: serum or plasma – 30 µL
Analytical sensitivity: 0.036 µg/L
Functional sensitivity (12.5%): 2.2 µg/L
|Normal adult values|
|Med µg/L||5. Perc. µg/L||95. Perc. µg/L||Min µg/L||Max µg/L|
A noninvasive method for monitoring kidney fibrosis and graft survival
State-of-the art techniques for evaluating liver fibrosis