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Procollagen-III-peptide (PIIINP) ELISA kit (CE) ELISA

The P3NP-ELISA test enables the determination of human procollagen III peptide in blood to monitor fibroses, reducing the number of necessary patient biopsies.
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The P3NP-ELISA test enables the determination of human procollagen III peptide in blood to monitor fibroses, reducing the number of necessary patient biopsies.
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In stock

Overview

Detect the transformation of functioning liver tissue into connective tissue (in alcoholic or virus induced forms of liver fibrosis, …)

Detect and monitor the course of liver disorders (fibrosis and cirrhosis), in particular psoriatic patients treated by MTX and non-alcoholic fatty liver damage (NAFLD)

Reduce the number of biopsies for improved patient comfort and cost savings.

Detect lung fibrosis, chronic polyarthritis, collagenosis, myocardial infection, acromegaly and multiple traumas

Monitor disease progression

Benefits

  • ONE-STEP ELISA ASSAY AT R.T.
  • AUTOMATION FRIENDLY

Assay principle: sandwich

A monoclonal antibody, immobilized on the microplate, captures the PIIINP proteins contained in the calibrators and samples. The bound proteins are then recognized by a second monoclonal antibody conjugated to HRP (Horse-Radish peroxidase). The unbound reagents are eliminated by washing. Then, the colorimetric reaction is started by the addition of the HRP substrate TMB and stopped by addition of an acid solution. The optical density (OD) of each well is read at 450 nm. A predilution at 1/11 is required for controls and samples.

Assay procedure: 3h at RT / shaking 700rpm + 15 min at RT.

PIIINP kit Flow Chart

PIIINP kit Flow Chart

Features

Working range: 0.036 – 30 µg/L

Sample: serum or plasma – 30 µL

Analytical sensitivity: 0.036 µg/L

Functional sensitivity (12.5%): 2.2 µg/L

Normal values

Normal adult values
n=120

Mean µg/L

Med µg/L 5. Perc. µg/L 95. Perc. µg/L Min µg/L Max µg/L
4.9 4.6 2.9 8.1 2.1 13.1

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PIIINP ELISA kit

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P3NP-ELISA - Safety Data Sheet

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