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PolyGT-Biotin substrate HTRF®

PolyGT-Biotin has been shown to be a substrate for tyrosine-specific protein kinases.
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  • No-wash No-wash
  • Ease-of-use Ease-of-use
  • Highly specific Highly specific
PolyGT-Biotin has been shown to be a substrate for tyrosine-specific protein kinases.
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Overview

Poly-(Glu,Tyr) (4:1) is a random copolymer which has been shown to be a substrate for tyrosine-specific protein kinases. Poly-(GT)-biotin may be used with Streptavidin-XL665 and Anti-phosphotyrosine-cryptate for easily optimized HTRF® tyrosine kinase assays. HTRF enables to perform assay at physiological ATP concentrations.

Benefits

  • NO LIMITATION IN ATP CONCENTRATIONS
  • RAPID ASSAY SETUP
  • PHOSHO-(TYR) SPECIFIC

Assay principles

HTRF Tyrosine kinase assay use typically a combination of a Europium-cryptate labeled anti-phosphoresidue antibody, SA-XL665 and PolyGT-Biotin as substrate. The signal is proportional to the concentration of phospho-tyrosine.
HTRF kinase assay principle

Assay protocol

HTRF tyrosine kinase assay have two basic phases: 1. Enzymatic step: biotinylated PolyGT is incubated with the kinase of interest and with the compounds in presence of co-factors. The reaction starts with the addition of ATP. 2. Detection step: The phosphorylated biotinylated PolyGT is then detected by the addition of Streptavidin-XL665 and a Europium-cryptate labeled anti-phosphotyrosine antibody (MAb PY20-Eu cryptate or MAb PT66-Eu cryptate) prepared in a buffer containing EDTA to stop the enzymatic reaction.
HTRF kinase assay protocol

Open R&D: Sanofi Access Platform

In collaboration with Sanofi - Scientific Presentations

Experience the freedom of building your own assay

HTRF kinase activity toolbox reagents - Flyers

Product Insert Poly GT / 61GT0BLA-61GT0BLD

61GT0BLA-61GT0BLD - Product Insert

HTRF Product Catalog 2020 July update

All your HTRF assays in one document! - Catalog

A guide to Homogeneous Time Resolved Fluorescence

General principles of HTRF - Guides

The essential white paper to expand your knowledge on kinases

Learn about cutting edge kinase research in this free white paper - Guides

How HTRF compares to Western Blot and ELISA

Get the brochure about technology comparison. - Brochures

Revolutionize your kinases investigations with HTRF

Watch this video to learn more about kinases investigations - Videos

Easy method for kinetic binding determination

How to revolutionize your kinetic binding demonstration with HTRF kinase binding platform assays - Application Notes

Safety Data Sheet Poly GT / 61GT0BLA

61GT0BLA - Safety Data Sheet

Safety Data Sheet Poly GT / 61GT0BLD

61GT0BLD - Safety Data Sheet

Plate Reader Requirement

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