A method for determining enzyme kinetic parameters (Km, Kcat) using HTRF...
PAb Anti-phospho PKC substrate-Eu cryptate HTRF®
- High affinity
The Anti-phospho PKA-Eu cryptate is a labelled polyclonal antibody. It recognizes phosphorylated serine with arginine or lysine residues in the -2 and +2 positions and a hydrophobic residue in the +1 position. It does not cross-react with non-phosphorylated serine residue or phosphorylated serine in a different configuration, nor does it recognize phospho threonine. This allows the construction of a highly specific biochemical kinase assay. Any kind of substrate can be used, from small synthetic peptides to natural substrates, meaning high assay detection versatility. HTRF allows to perform assay at physiological ATP concentrations.
- NO LIMITATION IN ATP CONCENTRATIONS
- ANY KIND OF SUBSTRATES
- PHOSHO-(SER) SPECIFIC
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How to revolutionize your kinetic binding demonstration with HTRF kinase binding platform assays - Application Notes
Plate Reader Requirement
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