MAb Anti-phospho p53-Eu cryptate
Anti;phospho p53;Eu cryptate to detect phospho;p53;Ser15.
The Anti-phospho MEK1/2 substrate-Eu cryptate is a labeled polyclonal antibody enabling the detection of MEK1/2 when phosphorylated at Ser217/221. It recognizes singly phosphorylated MEK1/2 at Ser 217 but not MEK1/2 singly phosphorylated at Ser 221. It does not detect the non-phosphorylated MEK1/2. This allows the construction of a specific biochemical kinase assay. Any kind of substrate can be used, from small synthetic peptides to natural substrates, meaning high assay detection versatility. HTRF allows to perform assay at physiological ATP concentrations.
|Assay Format||Apparent ATP Km(µM)|
|COT/MBP protein direct33P||384 ± 55|
|COT/MEK protein direct HTRF||279 ± 40|
|COT/MEK peptide direct HTRF||268 ± 21|
61P17KAE-61P17KAZ-61P17KAY - Product Insert
Learn about cutting edge kinase research in this free white paper - Guides
Get the brochure about technology comparison. - Brochures
Watch this video to learn more about kinases investigations - Videos
How to revolutionize your kinetic binding demonstration with HTRF kinase binding platform assays - Application Notes
A method for determining enzyme kinetic parameters (Km, Kcat) using HTRF...
HTRF used for the foundation of a cellular Ser/Thr-kinase platform that ...