A method for determining enzyme kinetic parameters (Km, Kcat) using HTRF...
PAb Anti-phospho AKT substrate-Eu cryptate HTRF®
- High affinity
The Anti-phospho AKT substrate-Eu cryptate is a labeled polyclonal antibody enabling the detection of Akt when phosphorylated at Ser or Thr preceded by arginine or lysine in -5 and -3 positions. It does not cross-react with phosphorylated Ser or Thr in different configurations (slight cross reactions with -2 and +2 positions). It does not detect the non-phosphorylated Akt. This allows the construction of a specific biochemical kinase assay. Any kind of substrate can be used, from small synthetic peptides to natural substrates, meaning high assay detection versatility. HTRF allows to perform assay at physiological ATP concentrations.
- NO LIMITATION IN ATP CONCENTRATIONS
- ANY KIND OF SUBSTRATES
- PHOSHO-AKT (SER/THR) SPECIFIC
61P02KAE-61P02KAZ-61P02KAY - Product Insert
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How to revolutionize your kinetic binding demonstration with HTRF kinase binding platform assays - Application Notes
Plate Reader Requirement
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