Opioid Delta, Mu and KappaR fluorescent probe
Tag-lite Opioid Delta & Kappa receptors fluorescent probe
One of the major subtypes of opioid receptors, Mu Opioid (or MOP) is predominantly expressed in the brain. The Mu opioid receptor is highly involved in the nociception and stress systems. MOP ligands are most commonly used to treat acute and chronic pain, cough, digestive disorders, and psychiatric disorders characterized by reward dysfunction.
Cells expressing the MOP receptor are provided pre-labeled with Terbium, and can be used to conduct receptor binding studies on the aforementioned receptor.
Running your Mu opioid receptor binding assay using Tag-lite is as easy as it can get. Simply dispense 10 µL of labeled cells into each well, followed by 5 µL of labeled ligand (L0005RED) and 5 µL of the compound you wish to test. Like all HTRF assays, Tag-lite assays do not require any washing steps. A diagram of the procedure to be followed is given on the right.
A saturation binding assay measures total and non-specific binding for increasing concentrations of ligand under equilibrium conditions. To perform the assay, the fluorescent ligand is titrated into a solution containing a fixed amount of labeled cells, and then incubated to equilibrium. The HTRF ratio obtained from this titration is the total binding.
Examples of data obtained using Mu opioid labeled cells and their matching fluorescent ligand (L0005RED). Naltrindole was used as reference ligand. Results may vary from one HTRF® compatible reader to another.
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