Mouse Total SLP-76 cellular kit HTRF®
The Mouse Total SLP-76 kit detects cellular SLP-76, and can be used as a normalization assay with our Mouse phospho-SLP-76 kit to assist in the optimal monitoring of T-lymphocyte activation.
- High sensitivity
- Faster and more convenient than ELISA
The Mouse Total SLP-76 cellular assay kit is designed to monitor the expression level of SLP-76, whether phosphorylated or unphosphorylated. It is compatible with our Mouse phospho-SLP-76 (Ser376) kit, and enables the analysis of phosphorylated and total proteins from a single sample for better readouts of T-lymphocyte activation.
- DATA NORMALIZATION
Detection of mouse total SLP-76 with HTRF reagents can be performed in a single plate used for culturing, stimulation, and lysis. No washing steps are required. This HTS designed protocol enables miniaturization while maintaining robust HTRF quality.
Mouse EL-4 cells were plated in 6-well plates in complete culture medium and incubated at 37°C, 5% CO2. The same day, the cells were treated with 10 µg/mL of anti-CD3 antibody for 20 min, and then underwent a second treatment with 10 µg/mL of IgG with and without 11mM of H2O2 for 15min. After this treatment, cells were then lysed with supplemented lysis buffer #1 for 30 minutes at RT under gentle shaking, and 16 µL of lysate were transferred twice over into a low volume white microplate before the addition of 4 µL of the premixed HTRF mouse phospho-SLP76 (Ser376) or mouse Total-SLP76 detection reagents. The HTRF signal was recorded after ON incubation.
Physiologically relevant results fo fast flowing research - Flyers
Insider Tips for successful sample treatment - Technical Notes
HTRF and WB compatible guidelines - Technical Notes
Protocol for tumor xenograft analysis with HTRF - Technical Notes
Mastering the art of cell signaling assays optimization - Guides
Multi-tissue cellular modeling and anlysis of insulin signaling - Posters
A solution for phospho-protein analysis in metabolic disorders - Posters
Detailed protocol and direct comparison with WB - Posters
A single technology for 2D cells, 3D cells, and xenograft models - Posters
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Analysis of a large panel of diverse biological samples and cellular models - Posters
One technology across all samples - Application Notes
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Valuable guidelines for efficiently analyzing and interpreting results - Application Notes
Increased flexibility of phospho-assays - Application Notes
Analyse of PI3K/AKT/mTor translational control pathway - Application Notes
In collaboration with Bayer - Scientific Presentations
A fun video introducing you to phosphorylation assays with HTRF - Videos
Get the brochure about technology comparison. - Brochures
Plate Reader Requirement
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