Mouse Total SLP-76 cellular kit HTRF®
The Mouse Total SLP-76 kit detects cellular SLP-76, and can be used as a normalization assay with our Mouse phospho-SLP-76 kit to assist in the optimal monitoring of T-lymphocyte activation.
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Ready-to-use
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High sensitivity
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Faster and more convenient than ELISA
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No-wash
Overview
The Mouse Total SLP-76 cellular assay kit is designed to monitor the expression level of SLP-76, whether phosphorylated or unphosphorylated. It is compatible with our Mouse phospho-SLP-76 (Ser376) kit, and enables the analysis of phosphorylated and total proteins from a single sample for better readouts of T-lymphocyte activation.
Benefits
- SPECIFICITY
- PRECISION
- DATA NORMALIZATION
Total-SLP-76 assay principle
Total-SLP-76 2-plate assay protocol
Total-SLP-76 1-plate assay protocol
Detection of mouse total SLP-76 with HTRF reagents can be performed in a single plate used for culturing, stimulation, and lysis. No washing steps are required. This HTS designed protocol enables miniaturization while maintaining robust HTRF quality.
TCR signaling stimulation with anti-CD3 antibodies in Jurkat cells
Mouse EL-4 cells were plated in 6-well plates in complete culture medium and incubated at 37°C, 5% CO2. The same day, the cells were treated with 10 µg/mL of anti-CD3 antibody for 20 min, and then underwent a second treatment with 10 µg/mL of IgG with and without 11mM of H2O2 for 15min. After this treatment, cells were then lysed with supplemented lysis buffer #1 for 30 minutes at RT under gentle shaking, and 16 µL of lysate were transferred twice over into a low volume white microplate before the addition of 4 µL of the premixed HTRF mouse phospho-SLP76 (Ser376) or mouse Total-SLP76 detection reagents. The HTRF signal was recorded after ON incubation.
Function and regulation of SLP-76
HTRF cellular phospho-protein assays
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How to run a cell based phospho HTRF assay
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Unleash the potential of your phosphorylation research with HTRF
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HTRF Product Catalog 2020 July update
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Product Insert SLP76 total (m) Kit / 63ADK110PEG-63ADK110PEH
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Assays for neurosciences research
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Plate Reader Requirement
Choosing the right microplate reader ensures you’ll get an optimal readout. Discover our high performance reader, or verify if your lab equipment is going to be compatible with this detection technology.
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