Human IL6 kit
No-wash kit to quantify released Human IL6
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The mouse STING WT binding assay is designed to select and characterize compounds that specifically bind mouse STING protein.
A fast and easy way to identify new binders to mouse STING WT.
Stimulator of interferon genes (STING), also known as transmembrane protein 173 (TMEM173), is a protein playing a major role in innate immunity. Upon intracellular cytosolic DNA release from pathogens such as viruses and bacteria, 2’-3’cGAMP binds to STING protein and triggers the secretion of type 1 interferon. Identifying new STING ligands is therefore a way to control immunity and fight autoinflammatory diseases.
The HTRF STING binding assay is a competitive assay format which uses d2-labeled STING ligand, a 6His tagged mouse STING protein, and an anti 6His Cryptate-labeled antibody. Your compound competes with the STING ligand-d2 and thereby prevents FRET from occurring. |
The Mouse STING binding assay can be run in a 96- or 384-well low volume white plate (20 µL final). As described here, samples or standards are dispensed directly into the assay plate, the mouse His-tagged STING protein is then added followed by the dispensing of the HTRF reagents: the anti 6His antibody labeled with Terbium cryptate and the STING ligand labeled with d2. The reagents labelled with HTRF fluorophores may be pre-mixed and added in a single dispensing step. No washing steps are needed. The protocol can be further miniaturized or upscaled by simply resizing each addition volume proportionally.
Various compounds known to be STING ligands were added to the assay.
2'3'cGAMP displays a high affinity for the mouse protein in the nM range. The other bacterial compounds, cyclic di-AMP and cyclic di-GMP are in the tens nM range.
DMXAA and CMA, two well known compounds specific for mouse STING compete the labelled ligand, with a Ki in good correlation with the litterature.
STING, for STimulator of INterferon Genes, is a cytoplasmic homodimeric protein localized in the endoplasmic reticulum, and which plays an essential role in innate immunity. Upon pathogen infection or mitochrondrial shrinking during apoptosis, floating dsDNAs bind and activate a DNA sensor, cyclic GMP-AMP synthase (cGAS). Activated cGAS leads to the production of 2’-3’cGAMP, a cyclic dinucleotide, which then binds to STING proteins. In turn, phosphorylated STING interacts with TANK-binding-kinase-I (TBK1), leading to the recruitment and activation of the active interferon regulatory factor (IRF3) dimer. Nuclear translocation of the IRF3 dimer leads to the transcription of genes encoding IFN-α/β. In addition, the STING pathway controls NF-κB dependent inflammatory cytokine expression. As a negative feedback loop, the DNA-stimulated cGAS-STING-TBK1 pathway also triggers STING protein degradation through p62 SQSTM1 associated autophagy, to switch off IFNb production.
Best practices for pharmacological characterization of PPI inhibitors
Easy pharmacological characterization of PPI modulators. - Technical Notes
HTRF assays handle low- to high affinity protein-protein interactions
Deciphering low- and high affinity interactions - Application Notes
Nuclear receptor ligand identification with HTRF
Monitoring nuclear receptor binding with HTRF assays - Application Notes
HTRF addresses large protein-protein interaction complexes
Challenge large complexes with HTRF assays - Application Notes
HTRF PPI your dream assay served on a plate
Sandwiches aren't just for eating - Infographics
A brief history of Protein-Protein Interactions
How well do you know PPI? - Infographics
Virology research solutions using HTRF Protein-Protein Interaction assays
See how peer researchers challenge the viral life cycle with PPI assays - Application Notes
HTRF Product Catalog 2020 July update
All your HTRF assays in one document! - Catalog
A guide to Homogeneous Time Resolved Fluorescence
General principles of HTRF - Guides
How HTRF compares to Western Blot and ELISA
Get the brochure about technology comparison. - Brochures
Product Insert Bioch. STING (m) kit / 64BDSTGMPEG-64BDSTGMPEH
64BDSTGMPEG-64BDSTGMPEH - Product Insert
Innovative solutions in Virology research
Kits and reagents for virology research - Flyers
Safety Data Sheet (DEU) Bioch. STING (m) kit / 64BDSTGMPEG
64BDSTGMPEG - Safety Data Sheet
Safety Data Sheet (ELL) Bioch. STING (m) kit / 64BDSTGMPEG
64BDSTGMPEG - Safety Data Sheet
Safety Data Sheet (FRA-FR) Bioch. STING (m) kit / 64BDSTGMPEG
64BDSTGMPEG - Safety Data Sheet
Safety Data Sheet (ITA) Bioch. STING (m) kit / 64BDSTGMPEG
64BDSTGMPEG - Safety Data Sheet
Safety Data Sheet (SPA) Bioch. STING (m) kit / 64BDSTGMPEG
64BDSTGMPEG - Safety Data Sheet
Safety Data Sheet (ENG-GB) Bioch. STING (m) kit / 64BDSTGMPEG
64BDSTGMPEG - Safety Data Sheet
Safety Data Sheet (ENG-US) Bioch. STING (m) kit / 64BDSTGMPEG
64BDSTGMPEG - Safety Data Sheet
Safety Data Sheet (DEU) Bioch. STING (m) kit / 64BDSTGMPEH
64BDSTGMPEH - Safety Data Sheet
Safety Data Sheet (ELL) Bioch. STING (m) kit / 64BDSTGMPEH
64BDSTGMPEH - Safety Data Sheet
Safety Data Sheet (FRA-FR) Bioch. STING (m) kit / 64BDSTGMPEH
64BDSTGMPEH - Safety Data Sheet
Safety Data Sheet (ITA) Bioch. STING (m) kit / 64BDSTGMPEH
64BDSTGMPEH - Safety Data Sheet
Safety Data Sheet (SPA) Bioch. STING (m) kit / 64BDSTGMPEH
64BDSTGMPEH - Safety Data Sheet
Safety Data Sheet (ENG-GB) Bioch. STING (m) kit / 64BDSTGMPEH
64BDSTGMPEH - Safety Data Sheet
Safety Data Sheet (ENG-US) Bioch. STING (m) kit / 64BDSTGMPEH
64BDSTGMPEH - Safety Data Sheet
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