Cynomolgus TNF alpha kit
No-wash kit to quantify released Cynomolgus TNF alpha
The HTRF mouse IL2 kit is designed for the quantification of mouse IL2 release in cell supernatant.
IL2's major role is to modulate cell-mediated immunity through its effect on T cells. IL2 promotes the differentiation of T cells into effector or memory T cells, thus helping the body fight off infection. IL2 is also a key player in immuno-oncology since it is the main promoter of the differentiation of T cells into Treg (regulatory T cells).
Assessment of serum samples often requires enhanced sensitivity. In some cases, AlphaLISA assays may have sufficient sensitivity to enable the detection of low levels of analytes in serum or plasma. Check out the Mouse IL-2 AlphaLISA kit ’s analytical performances for more information or learn more about AlphaLISA. When assaying, always follow the recommended protocol and avoid highly haemolyzed samples.
The 4 Parameter Logistic (4PL) curve is commonly recommended for fitting an ELISA standard curve. This regression enables the accurate measurement of an unknown sample across a wider range of concentrations than linear analysis, making it ideally suited to the analysis of biological systems like cytokine releases.
To fully understand how to deal with HTRF data processing and also 4PL 1/y² fitting, please visit this page.
Cisbio also worked with Myassays.com to help you in your data analysis. By clicking on this link, you’ll be able to access a free online software to run your IL2 analysis.
|Sample size||16 µL|
|Final assay volume||20 µL|
|Kit components||Lyophilized standard, frozen detection antibodies, buffers &protocol.|
|LOD &LOQ (in Diluent)||12 pg/mL &35 pg/mL|
|Range||35 2,560 pg/mL|
|Time to result||Overnight at RT|
|Sample||Mean [IL2] (pg/mL)||CV|
Each of the 3 samples was measured 24 times, and % CV was calculated for each sample.
|Sample||[IL2] (pg/mL)||Mean (delta R)||CV|
Each of the samples was measured in 4 different experiments, and % CV was calculated for each sample.
In collaboration with CNRS - Scientific Presentations
In collaboration with Novartis - Scientific Presentations
In collaboration with Molecular Devices - Application Notes
In collaboration with Blood assay solution - Application Notes
Perform and optimize cytokine assays on PBMC - Technical Notes
A fun video introducing you to cytokines assays with HTRF - Videos
This leaflet presents the analytical performances of each assay - Brochures
Get the brochure about technology comparison. - Brochures
Choosing the right microplate reader ensures you’ll get an optimal readout. Discover our high performance reader, or verify if your lab equipment is going to be compatible with this detection technology.Let's find your reader