Mouse IL2 kit

The HTRF mouse IL2 kit is designed for the quantification of mouse IL2 release in cell supernatant.

No-wash
Low sample consumption
Under 2h bench time

The HTRF mouse IL2 kit is designed for the quantification of mouse IL2 release in cell supernatant.

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Overview

IL2's major role is to modulate cell-mediated immunity through its effect on T cells. IL2 promotes the differentiation of T cells into effector or memory T cells, thus helping the body fight off infection. IL2 is also a key player in immuno-oncology since it is the main promoter of the differentiation of T cells into Treg (regulatory T cells).

Assessment of serum samples often requires enhanced sensitivity. In some cases, AlphaLISA assays may have sufficient sensitivity to enable the detection of low levels of analytes in serum or plasma. Check out the Mouse IL-2 AlphaLISA kit ’s analytical performances for more information or learn more about AlphaLISA. When assaying, always follow the recommended protocol and avoid highly haemolyzed samples.

Benefits

SCALABLE FROM 96 TO 1536WELLS
T CELL ACTIVATION READOUT

Assay principle

Cell supernatant, sample, or standard is dispensed directly into the assay plate for the detection by HTRF® reagents (384-well low-volume white plate or Cisbio low-volume 96-well plate in 20 µl). The antibodies labeled with the HTRF donor and acceptor are pre-mixed and added in a single dispensing step, to further streamline the assay procedure. The assay can be run up to a 1536-well format by simply resizing each addition volume proportionally.

Assay data analysis

The 4 Parameter Logistic (4PL) curve is commonly recommended for fitting an ELISA standard curve. This regression enables the accurate measurement of an unknown sample across a wider range of concentrations than linear analysis, making it ideally suited to the analysis of biological systems like cytokine releases.

To fully understand how to deal with HTRF data processing and also 4PL 1/y² fitting, please visit this page.

Cisbio also worked with Myassays.com to help you in your data analysis. By clicking on this link, you’ll be able to access a free online software to run your IL2 analysis.

Technical specifications of mouse IL2 kit

Sample size	16 µL
Final assay volume	20 µL
Kit components	Lyophilized standard, frozen detection antibodies, buffers &protocol.
LOD &LOQ (in Diluent)	12 pg/mL &35 pg/mL
Range	35 2,560 pg/mL
Time to result	Overnight at RT
Species	Mouse only

Intra and inter assay

Intra-assay (n=24)

Sample	Mean [IL2] (pg/mL)	CV
1	100	9%
2	900	3%
3	1800	7%
	Mean CV	6%

Each of the 3 samples was measured 24 times, and % CV was calculated for each sample.

Inter-assay (n=4)

Sample	[IL2] (pg/mL)	Mean (delta R)	CV
1	80	329	2%
2	320	1461	3%
3	1280	5980	8%
		Mean CV	4%

Each of the samples was measured in 4 different experiments, and % CV was calculated for each sample.

Can HTRF technology answer to different scientific questions? An academic with industrial standards point of view

In collaboration with CNRS - Scientific Presentations

Following T-cell activation and differenciation with HTRF reagents: IL-2, IFN-g and IL-22

In collaboration with Novartis - Scientific Presentations

HTRF Cytokine cell-based assays

Monitor immune regulation the simple way - Flyers

HTRF assays for Oncology and Inflammation

Signaling in the immune system - Brochures

Normalize HTRF cytokine assays with viability assay on Spectramax I3X-Minimax

In collaboration with Molecular Devices - Application Notes

HTRF measurement of cytokine release from fresh blood samples

In collaboration with Blood assay solution - Application Notes

Cytokine secretion profile on PBMCs

One sample to measure them all - Posters

Guidelines from PBMC isolation to cytokine assay optimisation

Perform and optimize cytokine assays on PBMC - Technical Notes

How to freeze PBMC

Guideline for PBMC freezing - Technical Notes

How to measure multiple cytokines from 1 sample

What to expect at the bench - Videos

Product Insert IL2 (m) Kit / 62MIL02PEG-62MIL02PEH

62MIL02PEG-62MIL02PEH - Product Insert

HTRF makes cytokine detection easy

A fun video introducing you to cytokines assays with HTRF - Videos

HTRF Product Catalog 2019

All your HTRF assays in one document! - Catalog

A guide to Homogeneous Time Resolved Fluorescence

General principles of HTRF - Guides

Cytokine assays: a guide to success with HTRF

Setting up a successful cytokine assay - Guides

Portfolio of HTRF cytokine assays

This leaflet presents the analytical performances of each assay - Brochures

How HTRF compares to Western Blot and ELISA

Get the brochure about technology comparison. - Brochures

Safety Data Sheet (DEU) IL2 (m) Kit / 62MIL02PEG