Cynomolgus TNF alpha kit
No-wash kit to quantify released Cynomolgus TNF alpha
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The HTRF mouse IL2 kit is designed for the quantification of mouse IL2 release in cell supernatant.
IL2's major role is to modulate cell-mediated immunity through its effect on T cells. IL2 promotes the differentiation of T cells into effector or memory T cells, thus helping the body fight off infection. IL2 is also a key player in immuno-oncology since it is the main promoter of the differentiation of T cells into Treg (regulatory T cells).
Assessment of serum samples often requires enhanced sensitivity. In some cases, AlphaLISA assays may have sufficient sensitivity to enable the detection of low levels of analytes in serum or plasma. Check out the Mouse IL-2 AlphaLISA kit ’s analytical performances for more information or learn more about AlphaLISA. When assaying, always follow the recommended protocol and avoid highly haemolyzed samples.
The 4 Parameter Logistic (4PL) curve is commonly recommended for fitting an ELISA standard curve. This regression enables the accurate measurement of an unknown sample across a wider range of concentrations than linear analysis, making it ideally suited to the analysis of biological systems like cytokine releases.
To fully understand how to deal with HTRF data processing and also 4PL 1/y² fitting, please visit this page.
Cisbio also worked with Myassays.com to help you in your data analysis. By clicking on this link, you’ll be able to access a free online software to run your IL2 analysis.
Sample size | 16 µL |
Final assay volume | 20 µL |
Kit components | Lyophilized standard, frozen detection antibodies, buffers &protocol. |
LOD &LOQ (in Diluent) | 12 pg/mL &35 pg/mL |
Range | 35 2,560 pg/mL |
Time to result | Overnight at RT |
Species | Mouse only |
Intra-assay (n=24)
Sample | Mean [IL2] (pg/mL) | CV |
---|---|---|
1 | 100 | 9% |
2 | 900 | 3% |
3 | 1800 | 7% |
Mean CV | 6% |
Each of the 3 samples was measured 24 times, and % CV was calculated for each sample.
Inter-assay (n=4)
Sample | [IL2] (pg/mL) | Mean (delta R) | CV |
---|---|---|---|
1 | 80 | 329 | 2% |
2 | 320 | 1461 | 3% |
3 | 1280 | 5980 | 8% |
Mean CV | 4% |
Each of the samples was measured in 4 different experiments, and % CV was calculated for each sample.
In collaboration with CNRS - Scientific Presentations
Following T-cell activation and differenciation with HTRF reagents: IL-2, IFN-g and IL-22
In collaboration with Novartis - Scientific Presentations
HTRF Cytokine cell-based assays
Monitor immune regulation the simple way - Flyers
HTRF assays for Oncology and Inflammation
Signaling in the immune system - Brochures
Normalize HTRF cytokine assays with viability assay on Spectramax I3X-Minimax
In collaboration with Molecular Devices - Application Notes
HTRF measurement of cytokine release from fresh blood samples
In collaboration with Blood assay solution - Application Notes
Cytokine secretion profile on PBMCs
One sample to measure them all - Posters
Guidelines from PBMC isolation to cytokine assay optimisation
Perform and optimize cytokine assays on PBMC - Technical Notes
Guideline for PBMC freezing - Technical Notes
How to measure multiple cytokines from 1 sample
What to expect at the bench - Videos
Product Insert IL2 (m) Kit / 62MIL02PEG-62MIL02PEH
62MIL02PEG-62MIL02PEH - Product Insert
HTRF makes cytokine detection easy
A fun video introducing you to cytokines assays with HTRF - Videos
All your HTRF assays in one document! - Catalog
A guide to Homogeneous Time Resolved Fluorescence
General principles of HTRF - Guides
Cytokine assays: a guide to success with HTRF
Setting up a successful cytokine assay - Guides
Portfolio of HTRF cytokine assays
This leaflet presents the analytical performances of each assay - Brochures
How HTRF compares to Western Blot and ELISA
Get the brochure about technology comparison. - Brochures
Safety Data Sheet (DEU) IL2 (m) Kit / 62MIL02PEG
62MIL02PEG - Safety Data Sheet
Safety Data Sheet (ELL) IL2 (m) Kit / 62MIL02PEG
62MIL02PEG - Safety Data Sheet
Safety Data Sheet (FRA-FR) IL2 (m) Kit / 62MIL02PEG
62MIL02PEG - Safety Data Sheet
Safety Data Sheet (ITA) IL2 (m) Kit / 62MIL02PEG
62MIL02PEG - Safety Data Sheet
Safety Data Sheet (SPA) IL2 (m) Kit / 62MIL02PEG
62MIL02PEG - Safety Data Sheet
Safety Data Sheet (ENG-GB) IL2 (m) Kit / 62MIL02PEG
62MIL02PEG - Safety Data Sheet
Safety Data Sheet (ENG-US) IL2 (m) Kit / 62MIL02PEG
62MIL02PEG - Safety Data Sheet
Safety Data Sheet (DEU) IL2 (m) Kit / 62MIL02PEH
62MIL02PEH - Safety Data Sheet
Safety Data Sheet (ELL) IL2 (m) Kit / 62MIL02PEH
62MIL02PEH - Safety Data Sheet
Safety Data Sheet (FRA-FR) IL2 (m) Kit / 62MIL02PEH
62MIL02PEH - Safety Data Sheet
Safety Data Sheet (ITA) IL2 (m) Kit / 62MIL02PEH
62MIL02PEH - Safety Data Sheet
Safety Data Sheet (SPA) IL2 (m) Kit / 62MIL02PEH
62MIL02PEH - Safety Data Sheet
Safety Data Sheet (ENG-GB) IL2 (m) Kit / 62MIL02PEH
62MIL02PEH - Safety Data Sheet
Safety Data Sheet (ENG-US) IL2 (m) Kit / 62MIL02PEH
62MIL02PEH - Safety Data Sheet
Safety Data Sheet (DEU) IL2 (m) Kit / 62MIL02PEJ
62MIL02PEJ - Safety Data Sheet
Safety Data Sheet (ELL) IL2 (m) Kit / 62MIL02PEJ
62MIL02PEJ - Safety Data Sheet
Safety Data Sheet (FRA-FR) IL2 (m) Kit / 62MIL02PEJ
62MIL02PEJ - Safety Data Sheet
Safety Data Sheet (ITA) IL2 (m) Kit / 62MIL02PEJ
62MIL02PEJ - Safety Data Sheet
Safety Data Sheet (SPA) IL2 (m) Kit / 62MIL02PEJ
62MIL02PEJ - Safety Data Sheet
Safety Data Sheet (ENG-GB) IL2 (m) Kit / 62MIL02PEJ
62MIL02PEJ - Safety Data Sheet
Safety Data Sheet (ENG-US) IL2 (m) Kit / 62MIL02PEJ
62MIL02PEJ - Safety Data Sheet
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