The HTRF mouse IL17 kit is designed for the quantification of mouse IL17 release in cell supernatant.

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  • No-wash No-wash
  • Low sample consumption Low sample consumption
  • Under 2h bench time Under 2h bench time

The HTRF mouse IL17 kit is designed for the quantification of mouse IL17 release in cell supernatant.

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Overview

IL17 is a pro-inflammatory cytokine induced by IL23 and produced by helper T cells. IL17 is part of the response to fungi and bacteria, and promotes the release of other pro-inflammatory cytokines and chemokines. Due to its role as a mediator of inflammatory responses, IL17 has been linked to allergic responses and autoimmune diseases like asthma, psoriasis, or IBD (inflammatory bowel disease). In immuno-oncology, IL17 is the main cytokine released by differentiated TH17 cells.


Assessment of serum samples often requires enhanced sensitivity. In some cases, AlphaLISA assays may have sufficient sensitivity to enable the detection of low levels of analytes in serum or plasma. Check out the  Mouse IL-17A homodimer AlphaLISA kit  , the Mouse/rat IL-17A AlphaLISA kit or the  Mouse/rat IL-17A AlphaLISA biotin-free kit’s analytical performances for more information or learn more about AlphaLISA. When assaying, always follow the recommended protocol and avoid highly haemolyzed samples.

Benefits

  • SCALABLE FROM 96 TO 1536WELLS
  • TH17 ACTIVATION FUNCTIONAL READOUT

Assay principle

Cell supernatant, sample, or standard is dispensed directly into the assay plate for the detection by HTRF® reagents (384-well low-volume white plate or Cisbio low-volume 96-well plate in 20 µl). The antibodies labeled with the HTRF donor and acceptor are pre-mixed and added in a single dispensing step, to further streamline the assay procedure. The assay can be run up to a 1536-well format by simply resizing each addition volume proportionally.
IL17 kit assay principle

Assay data analysis

The 4 Parameter Logistic (4PL) curve is commonly recommended for fitting an ELISA standard curve. This regression enables the accurate measurement of an unknown sample across a wider range of concentrations than linear analysis, making it ideally suited to the analysis of biological systems like cytokine releases.

To fully understand how to deal with HTRF data processing and also 4PL 1/y² fitting, please visit this page.

Cisbio also worked with Myassays.com to help you in your data analysis. By clicking on this link, you’ll be able to access a free online software to run your IL17 analysis.

Technical specifications of mouse IL17 kit

Sample size 16 µL
Final assay volume 20 µL
Kit components Lyophilized standard, frozen detection antibodies, buffers &protocol.
LOd &LOQ (in Diluent) 4 pg/mL &16 pg/mL
Range 16 – 4,000 pg/mL
Time to result 1h at RT
Species Mouse only

Intra and inter assay

Intra-assay (n=24)

Sample Mean [IL17] (pg/mL) CV
1 25 18%
2 1724 3%
3 3448 4%
Mean CV 8%

Each of the 3 samples was measured 24 times, and % CV was calculated for each sample.

Inter-assay (n=4)

Sample [IL17] (pg/mL) Mean (delta R) CV
1 41 273 5%
2 256 1678 5%
3 1600 9074 7%
Mean CV 6%

Each of the samples was measured in 4 different experiments, and % CV was calculated for each sample.

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