Human Fc kit
Human Fc quantification for bioprocess applications
Mouse Fc kit HTRF®
The Mouse Fc kit is designed for the fast quantification of mouse IgG or mouse Fc tagged proteins.
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Ready-to-use
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Highly specific
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Faster and more convenient than ELISA
Overview
Drug discovery and bioprocess often require accurate quantification technologies to improve the productivity workflow. Current methods such as ELISA do not properly fit with such objectives as they require multiple sample-handling and protocol steps. The Mouse Fc kit enables a rapid quantification of mouse IgG, and any proteins containing a mouse Fc region. The assay is compatible with natural IgG as well as recombinant antibodies.
Benefits
- LOW SAMPLE CONSUMPTION
- BROAD DYNAMIC RANGE
Assay principle
The mouse Fc kit is a competitive immunoassay for the measurement of mIgGs or any mFc-tagged proteins in a range of biological materials. The assay uses an Eu3+-Cryptate conjugated antibody specific to mFc and XL665 coupled mIgGs. mFc contained in the sample competes with XL665-labelled mouse IgG for binding to anti-mouse Fc Eu Cryptate.
Assay Protocol
The mouse Fc assay features a streamlined protocol with only 1 incubation step after sample and Mouse Fc detection reagents dispensing.
This protocol requires a single two-hour incubation period.
Key features
| Mouse Fc | |
|---|---|
| Detection limit: | 6.8 ng/mL |
| Dynamic range: | 0.8 400,000 ng/mL |
| Incubation time: | 2h at RT |
| EC50: | 138 ng/mL |
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Product Insert Fc (m) Kit / 6FMIGPEH
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All your HTRF assays in one document! - Catalog
A guide to Homogeneous Time Resolved Fluorescence
General principles of HTRF - Guides
How HTRF compares to Western Blot and ELISA
Get the brochure about technology comparison. - Brochures
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Kits and reagents for virology research - Flyers
Safety Data Sheet Fc (m) Kit / 6FMIGPEH
6FMIGPEH - Safety Data Sheet
Plate Reader Requirement
Choosing the right microplate reader ensures you’ll get an optimal readout. Discover our high performance reader, or verify if your lab equipment is going to be compatible with this detection technology.
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