A method for determining enzyme kinetic parameters (Km, Kcat) using HTRF...
MAb Anti-phosphoSAP/JNK-Eu cryptate HTRF®
- High affinity
The Anti-phospho SAP/JNK-Eu cryptate is a labeled monoclonal antibody enabling the rapid detection of SAP/JNK only when phosphorylated at Thr183 & Tyr185. It does not cross-react with phosphorylated forms of p44/42 or p38 MAPK. It does not detect the non-phosphorylated SAP/JNK. This allows the construction of a highly specific biochemical kinase assay. Any kind of substrate can be used, from small synthetic peptides to natural substrates, meaning high assay detection versatility. As HTRF tolerates extremely high ATP concentrations, an assay can be performed at physiological ATP concentrations.
- NO LIMITATION IN ATP CONCENTRATIONS
- ANY KIND OF SUBSTRATES
- PHOSHO-SAP/JNK (THR183/TYR185) SPECIFIC
61P09KAE-61P09KAZ-61P09KAY - Product Insert
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How to revolutionize your kinetic binding demonstration with HTRF kinase binding platform assays - Application Notes
Plate Reader Requirement
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