A method for determining enzyme kinetic parameters (Km, Kcat) using HTRF...
MAb Anti-phospho I kappa B alpha-Eu cryptate HTRF®
- High affinity
The Anti-phospho I kappa B alpha-Eu cryptate is a labeled monoclonal antibody enabling the rapid detection of I Kappa B alpha only when phosphorylated at Ser32/36, it does not cross-react with the corresponding non-phosphorylated sequence. This allows the construction of a highly specific biochemical kinase assay. Any kind of substrate can be used, from small synthetic peptides to natural substrates, meaning high assay detection versatility. As HTRF tolerates extremely high ATP concentrations, an assay can be performed at physiological ATP concentrations.
- NO LIMITATION IN ATP CONCENTRATIONS
- ANY KIND OF SUBSTRATES
- PHOSHO-IKBALPHA (SER32/36) SPECIFIC
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How to revolutionize your kinetic binding demonstration with HTRF kinase binding platform assays - Application Notes
Plate Reader Requirement
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