A method for determining enzyme kinetic parameters (Km, Kcat) using HTRF...
MAb Anti-phospho histone H3-Eu cryptate HTRF®
- High affinity
The Anti-phospho histone H3-Eu cryptate is a labeled monoclonal antibody enabling the rapid detection of histone H3 only when phosphorylated at Ser10. It does not cross-react with the corresponding non-phosphorylated sequence. This allows the construction of a highly specific biochemical kinase assay. Any kind of substrate can be used, from small synthetic peptides to natural substrates, meaning high assay detection versatility. As HTRF tolerates extremely high ATP concentrations, an assay can be performed at physiological ATP concentrations.
- NO LIMITATION IN ATP CONCENTRATIONS
- ANY KIND OF SUBSTRATES
- PHOSHO-HISTONE H3 (SER10) SPECIFIC
61P07KAE-61P07KAZ-61P07KAY - Product Insert
Learn about cutting edge kinase research in this free white paper - Guides
Get the brochure about technology comparison. - Brochures
Watch this video to learn more about kinases investigations - Videos
How to revolutionize your kinetic binding demonstration with HTRF kinase binding platform assays - Application Notes
Plate Reader Requirement
Choosing the right microplate reader ensures you’ll get an optimal readout. Discover our high performance reader, or verify if your lab equipment is going to be compatible with this detection technology.Let's find your reader