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Leukotriene C4 kit HTRF®

The Leukotriene C4 kit is designed for the accurate quantification of LTC4.
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  • Ready-to-use Ready-to-use
  • Highly specific Highly specific
  • Faster and more convenient than ELISA Faster and more convenient than ELISA
The Leukotriene C4 kit is designed for the accurate quantification of LTC4.
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Overview

LTC4 is a major leukotriene product that is enzymatically derived from the arachidonic acid cascade. Leukotriene synthesis operates via the 5-lipoxygenase (5-LO) pathway in which 5-LO converts arachidonic acid into LTA4 with 5(S)-HpETE as an intermediate. The conjugation of glutathione to LTA4 results in the formation of LTC4, which is then rapidly metabolized to LTD4 and LTE4. LTC4 has been described as a potent mediator of inflammatory diseases and immunoregulation. The LTC4 kit enables the monitoring of LTC4 produced by cells and can be used to study the LTC4-hydrolase.

Benefits

  • CELLS AND CELL SUPERNATANT FORMATS
  • LTC4-HYDROLASE STUDY

Assay principle

The LTC4 kit is a competitive immunoassay for the measurement of LTC4 in a range of biological formats. The assay uses an Eu3+-Cryptate conjugated antibody specific to LTC4 and XL665 coupled LTC4. LTC4 contained in the sample competes with XL665-labelled LTC4 for binding to anti-LTC4 Eu Cryptate.
LTB4 kit assay principle

Assay Protocol

The LTC4 assay features a streamlined protocol with only 1 incubation step after sample and LTC4 detection reagents dispensing. This protocol requires a single two-hour incubation period at RT.
LTB4 kit assay protocol

Key features

LTC4
Incubation 1h at RT
Assay range 0.1 – 5000 ng/mL
EC50 2.7 ng/mL
Detection limit 0.1 ng/mL
Specificity No cross reactivity with LTA4, LTB4
LTC4 standard curve

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Product Insert LTC4 Kit / 64LC4PEG-64LC4PEH

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HTRF Product Catalog 2020 July update

All your HTRF assays in one document! - Catalog

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How HTRF compares to Western Blot and ELISA

Get the brochure about technology comparison. - Brochures

Plate Reader Requirement

Choosing the right microplate reader ensures you’ll get an optimal readout. Discover our high performance reader, or verify if your lab equipment is going to be compatible with this detection technology.

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