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Leukotriene B4 kit HTRF®

The Leukotriene B4 kit is designed for the accurate quantification of LTB4.
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  • Ready-to-use Ready-to-use
  • Highly specific Highly specific
  • Faster and more convenient than ELISA Faster and more convenient than ELISA
The Leukotriene B4 kit is designed for the accurate quantification of LTB4.
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Overview

LTB4 is a major leukotriene product that is enzymatically derived from the arachidonic acid cascade. LTB4 is synthesized by leukocytes via the 5-lipoxygenase pathway and mediates different leukocyte functions such as lysosomal enzyme release, adhesion, and aggregation of polymorphonuclear leukocytes. LTB4 has been described as a potent mediator of inflammatory diseases and immunoregulation. The LTB4 kit enables the monitoring of LTB4 produced by cells and can be used to study the LTA4-hydrolase.

Benefits

  • CELLS AND CELL SUPERNATANT FORMATS
  • LTA4-HYDROLASE STUDY

Assay principle

The LTB4 kit is a competitive immunoassay for the measurement of LTB4 in a range of biological formats. The assay uses an Eu3+-Cryptate conjugated antibody specific to LTB4 and XL665 coupled LTB4. LTB4 in the sample competes with XL665-labelled LTB4 for binding to anti-LTB4 Eu Cryptate.
LTB4 kit assay principle

Assay Protocol

The LTB4 assay features a streamlined protocol with only 1 incubation step after sample and LTB4 detection reagents dispensing. This protocol requires a single two-hour incubation period at RT.
LTB4 kit assay protocol

Key features

LTB4
Incubation 1h at RT
Assay range 0.15 – 166.67 ng/mL
EC50 1.12 ng/mL
Detection limit 0.15 ng/mL
Specificity Low cross reactivity with 6-trans 12 epi LTB4
LTB4 standard curve

HTRF assays for Oncology and Inflammation

Signaling in the immune system - Brochures

Product Insert LTB4 Kit / 64LB4PEG-64LB4PEH

64LB4PEG-64LB4PEH - Product Insert

HTRF Product Catalog 2020 July update

All your HTRF assays in one document! - Catalog

A guide to Homogeneous Time Resolved Fluorescence

General principles of HTRF - Guides

How HTRF compares to Western Blot and ELISA

Get the brochure about technology comparison. - Brochures

Plate Reader Requirement

Choosing the right microplate reader ensures you’ll get an optimal readout. Discover our high performance reader, or verify if your lab equipment is going to be compatible with this detection technology.

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