KRAS WT/SOS1 binding kit
KRAS WT/SOS1 biochemical binding assay
KRAS WT GTP binding kit HTRF®
The KRAS WT GTP binding kit is designed to identify KRAS WT / GTP inhibitors.
-
No-wash
-
All inclusive kit
-
Accurate pharmacology
Overview
A fast and easy way to identify new KRAS WT / GTP competitors.
KRAS is a small GTPase implicated in various biological processes, such as cell proliferation, cell survival, and cell metabolism. This proto-oncogene is well known to be mutated in many cancer subtypes, inducing an uncontrolled proliferation and cell metabolism modifications. It thereby contributes to the Warburg effect in cancer cells. Like the majority of small GTPases, KRAS binds to GDP in its inactive form or binds to GTP to switch to the active form. KRAS G12C is one of the most commonly present mutant forms in cancer which lead to a permanently active state of KRAS. Identifying new KRAS / GTP competitors is therefore a relevant strategy to control biological processes involved in cancer growth by reducing the KRAS activity, as well as the associated pathways.
Benefits
- CANCER RESEARCH
- KRAS INHIBITORS
GTP-Red reagent Kd | 70nM |
GTP-Red reagent concentration | 50nM |
KRAS GTP binding Standard (GDP) IC50 | 35nM |
KRAS GTP binding Standard Ki | 20.4nM |
Screening of GTP competitors with human KRAS WT protein
Various nucleotides known to be GTP competitors were added to the assay. GDP (assay standard), GTPyS, and GTP displayed the right potency, in good correlation with the literature. ATP, a non-specific nucleotide, and BAY-293, a KRAS/SOS1 inhibitor, had no effect on the assay, confirming the specificity of the kit.
Best practices for pharmacological characterization of PPI inhibitors
Easy pharmacological characterization of PPI modulators. - Technical Notes
HTRF assays handle low- to high affinity protein-protein interactions
Deciphering low- and high affinity interactions - Application Notes
Nuclear receptor ligand identification with HTRF
Monitoring nuclear receptor binding with HTRF assays - Application Notes
HTRF addresses large protein-protein interaction complexes
Challenge large complexes with HTRF assays - Application Notes
HTRF PPI your dream assay served on a plate
Sandwiches aren't just for eating - Infographics
A brief history of Protein-Protein Interactions
How well do you know PPI? - Infographics
Virology research solutions using HTRF Protein-Protein Interaction assays
See how peer researchers challenge the viral life cycle with PPI assays - Application Notes
Product Insert KRAS WT GTP Kit / 64BDKRASWPEG-64BDKRASWPEH
64BDKRASWPEG-64BDKRASWPEH - Product Insert
KRAS GTP competitors and KRAS/SOS1 inhibitors screening with new HTRF biochemical-based assays
Screening for KRAS competitors and inhibitors with HTRF technology - Application Notes
Plate Reader Requirement
Choosing the right microplate reader ensures you’ll get an optimal readout. Discover our high performance reader, or verify if your lab equipment is going to be compatible with this detection technology.
Let's find your reader
You might be interested in