Intact Parathyroid hormone immunoradiometric assay kit RIA

The ELSA-PTH kit is an immunoradiometric assay for intact parathyroid hormone. There is an alternative protocol for pre-operative use (surgical gland exeresis).

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The ELSA-PTH kit is an immunoradiometric assay for intact parathyroid hormone. There is an alternative protocol for pre-operative use (surgical gland exeresis).

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Overview

Evaluate calcium metabolism disorders

Distinguish primary causes of hyperparathyroidism caused by hyperplasia or hypersecreting adenoma of the parathyroid glands Distinguish secondary causes of hyperparathyroidism caused by renal deficiency or lack of vitamin D or calcium

Distinguish between paraneoplasic syndromes

Diagnosis hypoparathyroidism, either the primary idiopathic form with hypocalcaemia or the secondary form with hypercalcaemia

Benefits

ALTERNATIVE PROTOCOL PRE-OPERATIVE USE
EXCELLENT SENSITIVITY

Assay principle: sandwich

The antibodies used for the sandwich recognize the intact 84 amino acid biological chain of parathyroid hormone (PTH 1-84). The monoclonal antibodies, specific to the mid region and the C-terminal part of the 39-84 of the molecule, are adsorbed onto the ELSA solid phase. The antibodies radiolabeled with iodine-125 are polyclonals recognizing the N-terminal part 1-34 of the molecule used as the tracer.

Two alternative assay procedures:

1- 16-20h at RT

2- Rapid procedure: 30 min at RT / shaking.