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IA2 autoantibody (IA-2Ab) ELISA kit

IA2 autoantibody (IA-2Ab) ELISA kit ELISA

IA2AB-ELISA is an accurate enzymatic assay to measure Tyrosine phosphatase (IA2-Ab) Islet Antigen autoantibodies in human serum.

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IA2AB-ELISA is an accurate enzymatic assay to measure Tyrosine phosphatase (IA2-Ab) Islet Antigen autoantibodies in human serum.

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Overview

Autoantibodies against IA2-Ab are important pre-clinical markers of type I diabetes mellitus (insulin-dependent diabetes mellitus - IDDM)

Benefits

ACCURATE AND RELIABLE ENZYMATIC ASSAY

Assay principle: ELISA

IA2-Ab molecules are coated onto the ELISA plate wells. IA-2-biotin complexes constitute the liquid phase. IA2-Ab bind to the IA2-Ab coated onto the well, which will also interact with IA-2-biotin forming a bridge. Addition of streptavidin peroxidase (SA-POD) detects the bound IA-2-biotin. The unbound SA-POD in excess is washed off before the addition of the peroxidase substrate, TMB.

Assay procedure: 16-20h at 2-8°C + 1h at 2-8°C + 20 min at RT / shaking + 20 min at RT in the dark.