Human and mouse TGF beta 1 kit
Human and mouse TGF beta 1 kit standard
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TIMP1, also known as TIMP-1 (Tissue Inhibitor of Metallo Proteinases 1), is an endogenous extracellular inhibitor of MMPs (matrix metalloproteinases) belonging to the TIMP family, of which four isoforms are known (TIMP1 to TIMP4). TIMP1 plays a crucial role in Extracellular Matrix (ECM) composition, and is a key modulator of extracellular matrix degradation. It regulates matrix metalloproteinases (MMPs) by forming 1:1 complexes with MMPs (such as MMP-2 and MMP-9), and irreversibly inactivates them. In culture supernatants and biological fluids, TIMP1 exists either as the free form TIMP1 (uncomplexed), or as tight TIMP1-MMP complexes. TIMPs maintain the balance between matrix destruction and formation. A MMPs/TIMPs imbalance leads to excessive ECM deposition and to fibrogenic processes.
Sample size | 16 µL |
---|---|
Final assay volume | 20 µL |
Time to results | ON at RT |
Kit components | Frozen standard, frozen detection antibodies, buffers & protocol. |
LOD & LOQ (in Diluent) | 0.15 ng/mL & 0.63 ng/mL |
Range | 0.8 – 50 ng/mL |
Species | Human only |
Specificity | no cross-reactivity with human TIMP2 and TIMP3 |
Sample | Mean [TIMP1] (ng/mL) | CV |
---|---|---|
1 | 1.09 | 5.5% |
2 | 5.90 | 1.9% |
3 | 20.5 | 2.1% |
Mean CV | 3.7% |
Each of the 3 samples was measured 24 times, and the % CV was calculated for each sample.
Sample | TIMP1 (ng/mL) | Mean (delta ratio) | CV |
---|---|---|---|
1 | 0.89 | 114 | 12% |
2 | 6.6 | 1102 | 5% |
3 | 26.9 | 4666 | 3% |
6% |
Each of the samples was measured in 4 different experiments, and the % CV was calculated for each sample.
IMR90 (human fetal lung myofibroblast, 1.6 x106 cells) and LX2* (human hepatic stellate cells, 2.7 x106 cells) were respectively plated in T75 culture flasks (duplicates) in DMEM containing 10% FCS. After 3 days, the medium was changed to DMEM containing 0.2% BSA, and then the cells were either left unstimulated or stimulated for 3 days with TGFβ1 (10 ng/mL).The supernatants from each flask were collected and hTIMP1 was quantified after a 1/16 dilution in diluent from the kit following the hTIMP1 kit protocol.
*Provided by EMD Millipore (product number #SCC064)
A side-by-side comparison between the HTRF Human TIMP1 assay kit and a commercial ELISA was performed on cell supernatants of MRC5, IMR90, and LX-2* cells, either unstimulated or treated with TGF-β1 for 48h (in FBS-free medium + 0.2% BSA).The two methods are very well correlated, as demonstrated by the value of the correlation coefficient (r) which was close to 1.
*Provided by EMD Millipore (product number #SCC064)
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Safety Data Sheet (ENG-GB) TIMP1 (h) Kit / 64TIMPEG
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Safety Data Sheet (ELL) TIMP1 (h) Kit / 64TIMPEH
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Safety Data Sheet (FRA-FR) TIMP1 (h) Kit / 64TIMPEH
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Safety Data Sheet (ITA) TIMP1 (h) Kit / 64TIMPEH
64TIMPEH - Safety Data Sheet
Safety Data Sheet (SPA) TIMP1 (h) Kit / 64TIMPEH
64TIMPEH - Safety Data Sheet
Safety Data Sheet (ENG-GB) TIMP1 (h) Kit / 64TIMPEH
64TIMPEH - Safety Data Sheet
Safety Data Sheet (ENG-US) TIMP1 (h) Kit / 64TIMPEH
64TIMPEH - Safety Data Sheet
Safety Data Sheet (DEU) TIMP1 (h) Kit / 64TIMPEJ
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Safety Data Sheet (ELL) TIMP1 (h) Kit / 64TIMPEJ
64TIMPEJ - Safety Data Sheet
Safety Data Sheet (FRA-FR) TIMP1 (h) Kit / 64TIMPEJ
64TIMPEJ - Safety Data Sheet
Safety Data Sheet (ITA) TIMP1 (h) Kit / 64TIMPEJ
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Safety Data Sheet (SPA) TIMP1 (h) Kit / 64TIMPEJ
64TIMPEJ - Safety Data Sheet
Safety Data Sheet (ENG-GB) TIMP1 (h) Kit / 64TIMPEJ
64TIMPEJ - Safety Data Sheet
Safety Data Sheet (ENG-US) TIMP1 (h) Kit / 64TIMPEJ
64TIMPEJ - Safety Data Sheet
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