Human Pro-collagen type 1 kit HTRF®

The Human Pro-collagen type 1 kit is designed for the quantitative detection of pro-collagen in cell supernatants & whole cells.

Low sample consumption
Highly specific
Faster and more convenient than ELISA

Download the protocols

The Human Pro-collagen type 1 kit is designed for the quantitative detection of pro-collagen in cell supernatants & whole cells.

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Overview

Human pro-collagen type 1 is made by a combined association of two pro-alpha1 chains with a pro-alpha2 chain. These triple-stranded, rope-like pro-collagen molecules must be processed by enzymes outside the cell to form fibrils that cross-link to produce strong mature type I collagen fibers. The human pro-collagen type 1 kit is designed for the quantitative detection of human pro-collagen type 1, using cell supernatants, whole cells, or recombinant protein extracts.

Benefits

SUPERNATANTS & WHOLE CELLS COMPATIBLE

Assay principle

Human pro-collagen is measured using a sandwich immunoassay involving two specific anti-human pro-collagen antibodies, respectively labelled with Europium Cryptate (donor) and d2 (acceptor). The intensity of the signal is proportional to the concentration of pro-collagen present in the sample.

Assay Protocol

The human pro-collagen type 1 assay protocol is shown here using a 384-well small volume white plate (20 µL final). Cell supernatant, sample, or standard is dispensed directly into the assay plate for the detection of human pro-collagen by HTRF reagents. The antibodies labelled with HTRF fluorophores may be pre-mixed and added in a single dispensing step to further streamline the assay procedure. The assay can be run in 96- to 384-well plates by simply resizing each addition volume proportionally.

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63ADK014PEG-63ADK014PEH - Product Insert

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Safety Data Sheet (DEU) Pro-col. 1 (h) Kit / 63ADK014PEG

63ADK014PEG - Safety Data Sheet

Safety Data Sheet (ELL) Pro-col. 1 (h) Kit / 63ADK014PEG

63ADK014PEG - Safety Data Sheet

Safety Data Sheet (FRA-FR) Pro-col. 1 (h) Kit / 63ADK014PEG

63ADK014PEG - Safety Data Sheet

Safety Data Sheet (ITA) Pro-col. 1 (h) Kit / 63ADK014PEG

63ADK014PEG - Safety Data Sheet

Safety Data Sheet (SPA) Pro-col. 1 (h) Kit / 63ADK014PEG

63ADK014PEG - Safety Data Sheet

Safety Data Sheet (ENG-GB) Pro-col. 1 (h) Kit / 63ADK014PEG

63ADK014PEG - Safety Data Sheet

Safety Data Sheet (ENG-US) Pro-col. 1 (h) Kit / 63ADK014PEG

63ADK014PEG - Safety Data Sheet

Safety Data Sheet (DEU) Pro-col. 1 (h) Kit / 63ADK014PEH

63ADK014PEH - Safety Data Sheet

Safety Data Sheet (ELL) Pro-col. 1 (h) Kit / 63ADK014PEH

63ADK014PEH - Safety Data Sheet

Safety Data Sheet (FRA-FR) Pro-col. 1 (h) Kit / 63ADK014PEH

63ADK014PEH - Safety Data Sheet

Safety Data Sheet (ITA) Pro-col. 1 (h) Kit / 63ADK014PEH

63ADK014PEH - Safety Data Sheet

Safety Data Sheet (SPA) Pro-col. 1 (h) Kit / 63ADK014PEH

63ADK014PEH - Safety Data Sheet

Safety Data Sheet (ENG-GB) Pro-col. 1 (h) Kit / 63ADK014PEH

63ADK014PEH - Safety Data Sheet

Safety Data Sheet (ENG-US) Pro-col. 1 (h) Kit / 63ADK014PEH

63ADK014PEH - Safety Data Sheet

Safety Data Sheet (DEU) Pro-col. 1 (h) Kit / 63ADK014PEJ