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Human Pro-collagen type 1 kit HTRF®

The Human Pro-collagen type 1 kit is designed for the quantitative detection of pro-collagen in cell supernatants & whole cells.
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  • Low sample consumption Low sample consumption
  • Highly specific Highly specific
  • Faster and more convenient than ELISA Faster and more convenient than ELISA
The Human Pro-collagen type 1 kit is designed for the quantitative detection of pro-collagen in cell supernatants & whole cells.
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Overview

Human pro-collagen type 1 is made by a combined association of two pro-alpha1 chains with a pro-alpha2 chain. These triple-stranded, rope-like pro-collagen molecules must be processed by enzymes outside the cell to form fibrils that cross-link to produce strong mature type I collagen fibers. The human pro-collagen type 1 kit is designed for the quantitative detection of human pro-collagen type 1, using cell supernatants, whole cells, or recombinant protein extracts.

Benefits

  • SUPERNATANTS & WHOLE CELLS COMPATIBLE

Assay principle

Human pro-collagen is measured using a sandwich immunoassay involving two specific anti-human pro-collagen antibodies, respectively labelled with Europium Cryptate (donor) and d2 (acceptor). The intensity of the signal is proportional to the concentration of pro-collagen present in the sample.
Human pro-collagen type 1 kit principle

Assay Protocol

The human pro-collagen type 1 assay protocol is shown here using a 384-well small volume white plate (20 µL final). Cell supernatant, sample, or standard is dispensed directly into the assay plate for the detection of human pro-collagen by HTRF reagents. The antibodies labelled with HTRF fluorophores may be pre-mixed and added in a single dispensing step to further streamline the assay procedure. The assay can be run in 96- to 384-well plates by simply resizing each addition volume proportionally.
Human pro-collagen type 1 kit protocol

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Plate Reader Requirement

Choosing the right microplate reader ensures you’ll get an optimal readout. Discover our high performance reader, or verify if your lab equipment is going to be compatible with this detection technology.

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