Human LAG3/MHC II biochemical binding assay HTRF®

This immune checkpoint assay is designed to identify human LAG3/MHC II checkpoint inhibitors.
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  • All inclusive kit All inclusive kit
  • Scalable from 96 to 1536 format Scalable from 96 to 1536 format
  • Validated with reference Ab Validated with reference Ab
This immune checkpoint assay is designed to identify human LAG3/MHC II checkpoint inhibitors.
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The lymphocyte activation gene-3 (LAG-3), selectively transcribed in human activated T and NK cells, encodes a ligand for major histocompatibility complex (MHC) class II molecules. When LAG3 is bound to MHC II, T cell response is suppressed. Checkpoint inhibitors blocking LAG3/MHC II complex formation are generating considerable interest in cancer immunotherapy.



Assay principle

This LAG3/MHC II kit includes human recombinant immune checkpoint partners tagged for detection (LAG3 and MHC II), a reaction buffer, and labelled anti-tag antibodies for HTRF detection. Without treatment, LAG3 binds to MHC class II, and the binding of each detection antibody to its tag target generates an HTRF signal. In the presence of a compound or an anti-human blocking antibody, the LAG3/MHC class II interaction is disrupted and the HTRF signal decreases.
LAG3/MHC II kit assay principle

Inhibitory effect of blocking human and mouse reference Ab

The inhibitory effects of human and mouse anti-human LAG3 were tested at 10 nM LAG3 and 20 nM MHC II. Assay windows around 3 were obtained.
Inhibitory effect of blocking human and mouse anti-human reference Ab

LAG3MHCII binding assay

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Plate Reader Requirement

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