Human IL17 kit
No-wash kit to quantify released Human IL17
-->
The Human IL17A/IL17RA binding kit is designed to identify human IL17A/IL17RA inhibitors.
Interleukin 17A , a pro-inflammatory cytokine, belongs to a family of six cytokines; IL-17A, IL-17B, IL-17C, IL-17D, IL-E, and IL-17F. IL-17A binds a heterodimeric cell surface receptor (IL-17RA/IL-17RC), triggering a signaling cascade that results in NF-κB activation which leads to the stimulation of the pro-inflammatory cytokines IL-6 and IL-8. Although this cytokine exerts a protective role against many pathogens, the overproduction of IL-17A is associated with autoimmune diseases such as psoriasis, rheumatid arthritis, and Crohn's Disease.
The Human IL17RA/IL17RA binding kit can be run in 96- or 384-well low volume white plates (20 µL final). As described here, samples or standards are dispensed directly into the assay plate. The Tag1-IL17A protein and Tag2-IL17RA proteins are then added, followed by the dispensing of the HTRF reagents: the anti Tag1 reagent labeled with europium cryptate and the anti Tag2 antibody labeled with XL665. The reagents labeled with HTRF fluorophores may be pre-mixed and added in a single dispensing step. No washing steps are needed. The protocol can be further miniaturized or upscaled by simply resizing each addition volume proportionally.
The inhibitory effects of unlabeled IL17A and IL17R, and human anti-IL17A antibody, were tested. No inhibition was observed using an anti EGFR antibody (cetuximab), confirming the specificity of the kit for the study of IL17A/IL17RA interaction.
Best practices for pharmacological characterization of PPI inhibitors
Easy pharmacological characterization of PPI modulators. - Technical Notes
HTRF assays handle low- to high affinity protein-protein interactions
Deciphering low- and high affinity interactions - Application Notes
Nuclear receptor ligand identification with HTRF
Monitoring nuclear receptor binding with HTRF assays - Application Notes
HTRF addresses large protein-protein interaction complexes
Challenge large complexes with HTRF assays - Application Notes
HTRF PPI your dream assay served on a plate
Sandwiches aren't just for eating - Infographics
A brief history of Protein-Protein Interactions
How well do you know PPI? - Infographics
HTRF Product Catalog 2020 July update
All your HTRF assays in one document! - Catalog
A guide to Homogeneous Time Resolved Fluorescence
General principles of HTRF - Guides
How HTRF compares to Western Blot and ELISA
Get the brochure about technology comparison. - Brochures
Virology research solutions using HTRF Protein-Protein Interaction assays
See how peer researchers challenge the viral life cycle with PPI assays - Application Notes
Product Insert IL17A/IL17RA (h) Kit / 64BDIL17PEG-64BDIL17PEH
64BDIL17PEG-64BDIL17PEH - Product Insert
autoimmune diseases therapeutic approaches
Complete autoimmunity review in a single White Paper - Guides
Autoimmune diseases and therapeutic strategies
Check out our new guide featuring valuable insights on autoimmunity - Guides
Assays for autoimmunity research
Advance your research on autoimmune diseases - Flyers
Safety Data Sheet (DEU) IL17A/IL17RA (h) Kit / 64BDIL17PEG
64BDIL17PEG - Safety Data Sheet
Safety Data Sheet (ELL) IL17A/IL17RA (h) Kit / 64BDIL17PEG
64BDIL17PEG - Safety Data Sheet
Safety Data Sheet (FRA-FR) IL17A/IL17RA (h) Kit / 64BDIL17PEG
64BDIL17PEG - Safety Data Sheet
Safety Data Sheet (ITA) IL17A/IL17RA (h) Kit / 64BDIL17PEG
64BDIL17PEG - Safety Data Sheet
Safety Data Sheet (SPA) IL17A/IL17RA (h) Kit / 64BDIL17PEG
64BDIL17PEG - Safety Data Sheet
Safety Data Sheet (ENG-GB) IL17A/IL17RA (h) Kit / 64BDIL17PEG
64BDIL17PEG - Safety Data Sheet
Safety Data Sheet (ENG-US) IL17A/IL17RA (h) Kit / 64BDIL17PEG
64BDIL17PEG - Safety Data Sheet
Safety Data Sheet (DEU) IL17A/IL17RA (h) Kit / 64BDIL17PEH
64BDIL17PEH - Safety Data Sheet
Safety Data Sheet (ELL) IL17A/IL17RA (h) Kit / 64BDIL17PEH
64BDIL17PEH - Safety Data Sheet
Safety Data Sheet (FRA-FR) IL17A/IL17RA (h) Kit / 64BDIL17PEH
64BDIL17PEH - Safety Data Sheet
Safety Data Sheet (ITA) IL17A/IL17RA (h) Kit / 64BDIL17PEH
64BDIL17PEH - Safety Data Sheet
Safety Data Sheet (SPA) IL17A/IL17RA (h) Kit / 64BDIL17PEH
64BDIL17PEH - Safety Data Sheet
Safety Data Sheet (ENG-GB) IL17A/IL17RA (h) Kit / 64BDIL17PEH
64BDIL17PEH - Safety Data Sheet
Safety Data Sheet (ENG-US) IL17A/IL17RA (h) Kit / 64BDIL17PEH
64BDIL17PEH - Safety Data Sheet
Choosing the right microplate reader ensures you’ll get an optimal readout. Discover our high performance reader, or verify if your lab equipment is going to be compatible with this detection technology.
Let's find your readerYou might be interested in