Human G-CSF kit
No-wash kit to quantify released Human G-CSF
Human GM-CSF kit HTRF®
The HTRF human GM-CSF kit is designed for the quantification of human GM-CSF release in cell supernatant.
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No-wash
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Low sample consumption
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Under 2h bench time
Overview
Also known as CSF2 (colony-stimulating factor 2), GM-CSF (granulocyte-macrophage colony-stimulating factor) is a hematopoietic growth factor and an immune modulator. Under immune stimuli, it is produced by T lymphocytes, macrophages, endothelial cells, and fibroblasts. It acts on precursor cells in the bone marrow to initiate their proliferation and differentiation into mature granulocytes and macrophages. GM-CSF stimulates multipotent progenitor cells.
Assessment of serum samples often requires enhanced sensitivity. In some cases, AlphaLISA assays may have sufficient sensitivity to enable the detection of low levels of analytes in serum or plasma. Check out the Human GM-CSF AlphaLISA kit’s analytical performances for more information or learn more about AlphaLISA. When assaying, always follow the recommended protocol and avoid highly haemolyzed samples.
Benefits
- SCALABLE FROM 96 TO 1536WELLS
- NK CELL ACTIVATION READOUT
Assay principle
Cell supernatant, sample, or standard is dispensed directly into the assay plate for the detection by HTRF® reagents (384-well low-volume white plate or Cisbio low-volume 96-well plate in 20 µl). The antibodies labeled with the HTRF donor and acceptor are pre-mixed and added in a single dispensing step, to further streamline the assay procedure. The assay can be run up to a 1536-well format by simply resizing each addition volume proportionally.
Assay data analysis
The 4 Parameter Logistic (4PL) curve is commonly recommended for fitting an ELISA standard curve. This regression enables the accurate measurement of an unknown sample across a wider range of concentrations than linear analysis, making it ideally suited to the analysis of biological systems like cytokine releases.
To fully understand how to deal with HTRF data processing and also 4PL 1/y² fitting, please visit this page.
Cisbio also worked with Myassays.com to help you in your data analysis. By clicking on this link you’ll be able to access a free online software to run your GM-CSF analysis.
Technical specifications of human GM-CSF kit
| Sample size | 16 µL |
| Final assay volume | 20 µL |
| Kit components | Lyophilized standard, frozen detection antibodies, buffers & protocol. |
| LOD & LOQ (in Diluent) | 27 pg/mL & 47 pg/mL |
| Range | 47 – 4,500 pg/mL |
| Time to result | Overnight at RT |
| Calibration | NIBSC (09/136) value (IU/mL) = 0,07 x HTRF hGCSF HTRF |
| Species | Human only |
Intra and inter assay
Intra assay (n=24)
| Sample | Mean [GM-CSF] (pg/mL) | CV |
|---|---|---|
| 1 | 81 | 17% |
| 2 | 2125 | 4% |
| 3 | 6011 | 10% |
| Mean CV | 10% |
Inter assay (n=4)
| Sample | [GM-CSF] (pg/mL) | Mean (delta ratio) | |
|---|---|---|---|
| 1 | 169 | 370 | 6% |
| 2 | 774 | 1654 | 7% |
| 3 | 4135 | 5313 | 6% |
| Mean CV | 7% |
GM-CSF secretion by PBMC co-stimulated with ionomycin and PMA
PBMCs, plated at 100,000 cells/well in a 96 well plate, were stimulated for 24h with 1µg/ml of ionomycin and with various concentrations of PMA. 16µl of supernatant were transferred into a 384 well (small volume) white detection plate to be analyzed by the Human GM-CSF Assay.
In collaboration with CNRS - Scientific Presentations
HTRF Cytokine cell-based assays
Monitor immune regulation the simple way - Flyers
HTRF solutions for each cell type - Flyers
HTRF assays for Oncology and Inflammation
Signaling in the immune system - Brochures
Normalize HTRF cytokine assays with viability assay on Spectramax I3X-Minimax
In collaboration with Molecular Devices - Application Notes
HTRF measurement of cytokine release from fresh blood samples
In collaboration with Blood assay solution - Application Notes
Cytokine secretion profile on PBMCs
One sample to measure them all - Posters
Guidelines from PBMC isolation to cytokine assay optimisation
Perform and optimize cytokine assays on PBMC - Technical Notes
Guideline for PBMC freezing - Technical Notes
How to measure multiple cytokines from 1 sample
What to expect at the bench - Videos
Product Insert GM-CSF (h) Kit / 62HGMCSFPEG-62HGMCSFPEH
62HGMCSFPEG-62HGMCSFPEH - Product Insert
HTRF makes cytokine detection easy
A fun video introducing you to cytokines assays with HTRF - Videos
HTRF Product Catalog 2020 July update
All your HTRF assays in one document! - Catalog
A guide to Homogeneous Time Resolved Fluorescence
General principles of HTRF - Guides
Cytokine assays: a guide to success with HTRF
Setting up a successful cytokine assay - Guides
Portfolio of HTRF cytokine assays
This leaflet presents the analytical performances of each assay - Brochures
How HTRF compares to Western Blot and ELISA
Get the brochure about technology comparison. - Brochures
Safety Data Sheet (DEU) GM-CSF (h) Kit / 62HGMCSFPEG
62HGMCSFPEG - Safety Data Sheet
Safety Data Sheet (ELL) GM-CSF (h) Kit / 62HGMCSFPEG
62HGMCSFPEG - Safety Data Sheet
Safety Data Sheet (FRA-FR) GM-CSF (h) Kit / 62HGMCSFPEG
62HGMCSFPEG - Safety Data Sheet
Safety Data Sheet (ITA) GM-CSF (h) Kit / 62HGMCSFPEG
62HGMCSFPEG - Safety Data Sheet
Safety Data Sheet (SPA) GM-CSF (h) Kit / 62HGMCSFPEG
62HGMCSFPEG - Safety Data Sheet
Safety Data Sheet (ENG-GB) GM-CSF (h) Kit / 62HGMCSFPEG
62HGMCSFPEG - Safety Data Sheet
Safety Data Sheet (ENG-US) GM-CSF (h) Kit / 62HGMCSFPEG
62HGMCSFPEG - Safety Data Sheet
Safety Data Sheet (DEU) GM-CSF (h) Kit / 62HGMCSFPEH
62HGMCSFPEH - Safety Data Sheet
Safety Data Sheet (ELL) GM-CSF (h) Kit / 62HGMCSFPEH
62HGMCSFPEH - Safety Data Sheet
Safety Data Sheet (FRA-FR) GM-CSF (h) Kit / 62HGMCSFPEH
62HGMCSFPEH - Safety Data Sheet
Safety Data Sheet (ITA) GM-CSF (h) Kit / 62HGMCSFPEH
62HGMCSFPEH - Safety Data Sheet
Safety Data Sheet (SPA) GM-CSF (h) Kit / 62HGMCSFPEH
62HGMCSFPEH - Safety Data Sheet
Safety Data Sheet (ENG-GB) GM-CSF (h) Kit / 62HGMCSFPEH
62HGMCSFPEH - Safety Data Sheet
Safety Data Sheet (ENG-US) GM-CSF (h) Kit / 62HGMCSFPEH
62HGMCSFPEH - Safety Data Sheet
Safety Data Sheet (DEU) GM-CSF (h) Kit / 62HGMCSFPEJ
62HGMCSFPEJ - Safety Data Sheet
Safety Data Sheet (ELL) GM-CSF (h) Kit / 62HGMCSFPEJ
62HGMCSFPEJ - Safety Data Sheet
Safety Data Sheet (FRA-FR) GM-CSF (h) Kit / 62HGMCSFPEJ
62HGMCSFPEJ - Safety Data Sheet
Safety Data Sheet (ITA) GM-CSF (h) Kit / 62HGMCSFPEJ
62HGMCSFPEJ - Safety Data Sheet
Safety Data Sheet (SPA) GM-CSF (h) Kit / 62HGMCSFPEJ
62HGMCSFPEJ - Safety Data Sheet
Safety Data Sheet (ENG-GB) GM-CSF (h) Kit / 62HGMCSFPEJ
62HGMCSFPEJ - Safety Data Sheet
Safety Data Sheet (ENG-US) GM-CSF (h) Kit / 62HGMCSFPEJ
62HGMCSFPEJ - Safety Data Sheet
Plate Reader Requirement
Choosing the right microplate reader ensures you’ll get an optimal readout. Discover our high performance reader, or verify if your lab equipment is going to be compatible with this detection technology.
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