HTRF KinEASE STK Discovery kit
A assay to determine which HTRF KinEASE STK kit must be used
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The HTRF KinEASE kit series provides a simple biochemical approach to study kinase activity, screen for inhibitors and characterize them. Three STK and one TK kits, offer a semi-universal method for assessing phosphorylation on Serine/Threonine and Tyrosine residues, respectively. The HTRF KinEASE STK S3 assay has been validated on 43 Serine/Threonine kinases. The assay validation section of this page lists them and provides technical tips for setup. The kit series also include a Discovery kit to help determine which of three STK kits is appropriate when working with a non-validated enzyme.
As shown here, all HTRF KinEASE assays work the same way and involve two steps:
Step 1: Kinase reaction The kinase is incubated in the presence or absence of compounds and the appropriate substrate. The supplemented enzymatic buffer is added in the kinase buffer for tyr kinases only. ATP is added to start the reaction.
Step 2: Detection Eu-cryptate and XL665 conjugates are added. The detection buffer contains EDTA to stop the enzymatic reaction.
AKT1, PKB-alpha validated with HTRF KinEASE
Enzyme validation - Technical Notes
AKT2, PKB-beta validated with HTRF KinEASE
Enzyme validation - Technical Notes
AKT3, PKB-gamma validated with HTRF KinEASE
Enzyme validation - Technical Notes
ASK1 validated with HTRF KinEASE
Enzyme validation - Technical Notes
DYRK2 validated with HTRF KinEASE
Enzyme validation - Technical Notes
IKK-alpha, CHUK validated with HTRF KinEASE
Enzyme validation - Technical Notes
IKK-beta, IKBKB validated with HTRF KinEASE
Enzyme validation - Technical Notes
MSK2, RPS6KA4 validated with HTRF KinEASE
Enzyme validation - Technical Notes
NEK2 validated with HTRF KinEASE
Enzyme validation - Technical Notes
p70S6K, RPS6KB1 validated with HTRF KinEASE
Enzyme validation - Technical Notes
PIM1 validated with HTRF KinEASE
Enzyme validation - Technical Notes
PIM2 validated with HTRF KinEASE
Enzyme validation - Technical Notes
PKAC-gamma, PRKACG validated with HTRF KinEASE
Enzyme validation - Technical Notes
SGK1 validated with HTRF KinEASE
Enzyme validation - Technical Notes
SGK2 validated with HTRF KinEASE
Enzyme validation - Technical Notes
SGK3, SGKL validated with HTRF KinEASE
Enzyme validation - Technical Notes
Universal platform to address Ser/Thr & Tyr kinases - Application Notes
In collaboration with CNRS - Scientific Presentations
Open R&D: Sanofi Access Platform
In collaboration with Sanofi - Scientific Presentations
In collaboration with CNRS - Scientific Presentations
Progress towards a generic intracellular kinase assay
In collaboration with Genomics institute of the Novartis Research Foundation - Scientific Presentations
Biomarker and Cell Signaling Assays for Fibrosis and NASH
HTRF solutions for NASH - Flyers
KinEASE, a universal expanded platform to adress Ser/Thr & Tyr kinases
Measuring kinase activity under any conditions - Application Notes
Exploration is easier with a trusted partner
HTRF KinEASE Tyr and Ser/Thr kits - Flyers
Validated kinases and assay conditions - Flyers
Product Insert KinEASE STK S3 Kit / 62ST3PEB-62ST3PEC-62ST3PEJ
62ST3PEB-62ST3PEC-62ST3PEJ - Product Insert
HTRF Product Catalog 2020 July update
All your HTRF assays in one document! - Catalog
A guide to Homogeneous Time Resolved Fluorescence
General principles of HTRF - Guides
The essential white paper to expand your knowledge on kinases
Learn about cutting edge kinase research in this free white paper - Guides
How HTRF compares to Western Blot and ELISA
Get the brochure about technology comparison. - Brochures
Multiple therapeutic areas adressed with HTRF KinEase assays
Discover how peer researchers challenge kinase investigations with KinEase assays - Application Notes
Revolutionize your kinases investigations with HTRF
Watch this video to learn more about kinases investigations - Videos
Easy method for kinetic binding determination
How to revolutionize your kinetic binding demonstration with HTRF kinase binding platform assays - Application Notes
Safety Data Sheet KinEASE STK S3 Kit / 62ST3PEB
62ST3PEB - Safety Data Sheet
Safety Data Sheet (DEU) KinEASE STK S3 Kit / 62ST3PEB
62ST3PEB - Safety Data Sheet
Safety Data Sheet (ELL) KinEASE STK S3 Kit / 62ST3PEB
62ST3PEB - Safety Data Sheet
Safety Data Sheet (FRA-FR) KinEASE STK S3 Kit / 62ST3PEB
62ST3PEB - Safety Data Sheet
Safety Data Sheet (ITA) KinEASE STK S3 Kit / 62ST3PEB
62ST3PEB - Safety Data Sheet
Safety Data Sheet (SPA) KinEASE STK S3 Kit / 62ST3PEB
62ST3PEB - Safety Data Sheet
Safety Data Sheet (ENG-GB) KinEASE STK S3 Kit / 62ST3PEB
62ST3PEB - Safety Data Sheet
Safety Data Sheet (ENG-US) KinEASE STK S3 Kit / 62ST3PEB
62ST3PEB - Safety Data Sheet
Safety Data Sheet KinEASE STK S3 Kit / 62ST3PEC
62ST3PEC - Safety Data Sheet
Safety Data Sheet (DEU) KinEASE STK S3 Kit / 62ST3PEC
62ST3PEC - Safety Data Sheet
Safety Data Sheet (ELL) KinEASE STK S3 Kit / 62ST3PEC
62ST3PEC - Safety Data Sheet
Safety Data Sheet (FRA-FR) KinEASE STK S3 Kit / 62ST3PEC
62ST3PEC - Safety Data Sheet
Safety Data Sheet (ITA) KinEASE STK S3 Kit / 62ST3PEC
62ST3PEC - Safety Data Sheet
Safety Data Sheet (SPA) KinEASE STK S3 Kit / 62ST3PEC
62ST3PEC - Safety Data Sheet
Safety Data Sheet (ENG-GB) KinEASE STK S3 Kit / 62ST3PEC
62ST3PEC - Safety Data Sheet
Safety Data Sheet (ENG-US) KinEASE STK S3 Kit / 62ST3PEC
62ST3PEC - Safety Data Sheet
Safety Data Sheet KinEASE STK S3 Kit / 62ST3PEJ
62ST3PEJ - Safety Data Sheet
Safety Data Sheet (DEU) KinEASE STK S3 Kit / 62ST3PEJ
62ST3PEJ - Safety Data Sheet
Safety Data Sheet (ELL) KinEASE STK S3 Kit / 62ST3PEJ
62ST3PEJ - Safety Data Sheet
Safety Data Sheet (FRA-FR) KinEASE STK S3 Kit / 62ST3PEJ
62ST3PEJ - Safety Data Sheet
Safety Data Sheet (ITA) KinEASE STK S3 Kit / 62ST3PEJ
62ST3PEJ - Safety Data Sheet
Safety Data Sheet (SPA) KinEASE STK S3 Kit / 62ST3PEJ
62ST3PEJ - Safety Data Sheet
Safety Data Sheet (ENG-GB) KinEASE STK S3 Kit / 62ST3PEJ
62ST3PEJ - Safety Data Sheet
Safety Data Sheet (ENG-US) KinEASE STK S3 Kit / 62ST3PEJ
62ST3PEJ - Safety Data Sheet
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