HTRF 96-well low volume white plate HTRF®
All the benefits of HTRF with ELISA plate ease of use and low volume wells for low sample consumption. These 96w low volume plates can be used with all HTRF assays.
Overview
These plates have been specifically designed by Cisbio as an alternative to 384-well lv plate for small sample series or simply to try HTRF in a convenient environment. Cisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. They can be used in a no-transfer protocol with suspension cells or with a transfer step for adherent cells. These plates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy.
Benefits
- SMALL SAMPLE SERIES
- FIRST TRIALS WITH HTRF
- COMPATIBLE WITH ALL HTRF ASSAYS
Plate characteristics
Format | 96 well |
---|---|
Color | White |
Bottom | Opaque |
Maximum volume / well | 40µl |
Recommended working volume / well | 20-30µl |
Culture | NOT culture treated |
Lid | Compatible |
Sealer | Compatible (and recommended) |
Reader settings | 96 half-area (except Envision) |
Validation | Inter-batch &intra-batch |
1. cAMP cell based assay in 384 well and Cisbio 96 well plates
S/B | ||||
---|---|---|---|---|
Cells/w | 1000 | 2000 | 3000; | 4000 |
384 well lv plate | 2.1 | 2.8 | 3.2 | 3.4 |
HTRF 96 well lv plate | 2.1 | 2.5 | 2.9 | 3.4 |
EC50 (nM) | ||||
---|---|---|---|---|
Cells/w | 1000 | 2000 | 3000 | 4000 |
384 well lv plate | 4.4 | 3.6 | 2.9 | 2.4 |
HTRF 96 well lv plate | 6.7 | 4.8 | 3.3 | 3.0 |
2. ERK phosphorylation assay
HTRF 96- and 384- well low volume plates
Specifically designed by Cisbio to be used with all HTRF assays - Flyers
HTRF Product Catalog 2020 July update
All your HTRF assays in one document! - Catalog
A guide to Homogeneous Time Resolved Fluorescence
General principles of HTRF - Guides
HTRF 96-well low volume white plate
Characteristics of the HTRF 96-well low volume plate - Technical Notes
How HTRF compares to Western Blot and ELISA
Get the brochure about technology comparison. - Brochures
Review of cell-based methods to monitor CAR-T activation and cell killing effectiveness
A novel CAR-T cell therapy based on chimeric antigen receptors for tumor cells, that target CD19 antigens. - Application Notes
Plate Reader Requirement
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