HaloTag-Green labeling reagent HTRF®

SNAP-Tag, Clip-Tag, and HALO-Tag are protein labeling systems enabling the covalent attachement of fluorophores onto proteins of interest. HaloTag-Green enables HALO-tagged proteins to become labeled with a green fluorophore.

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Overview

Over the past few years, SNAP-Tag, CLIP-Tag, and Halo-Tag technologies combined with TR-FRET have paved the road to the development of many non-radioactive, no-wash binding assays. The method is based on transfecting cells using plasmids encoding a SNAP-Tag (or CLIP-Tag, or Halot-Tag) and subsequently labeling them with acceptors or donors. HaloTag-Green allows the labeling of Halo-Tagged proteins with a green fluorophore.

Benefits

COVALENT LABELING
FAST REACTION

Receptor labeling

HALO-tag is a small fusion tag that covalently interacts with specific substrates. HALO-tag enables the specific and covalent labeling of any protein of interest. Contact your local scientific consultant to receive more information on HALO-Tag and its applications.

Evaluation of a Tag-lite binding assay for a class B receptor

In collaboration with Boehringer Ingelheim - Scientific Presentations

Ultra HTS at Bayer: use of IP-One and Tag-lite assays in GPCR drug discovery

In collaboration with Bayer - Scientific Presentations

Determination of association (kon) and dissociation (koff) rates constants using the Tag-lite platform

Challenge the limits of binding kinetics studies - Application Notes

Understanding GPCRs is the key to improved DD

In collaboration with GEN - Guides

Tag-lite, the easiest solution for studying GPCR-ligands interactions

The gold standard technology for receptor binding studies - Videos

Product Insert Halo-Green Subst / SHALOGRNE

SHALOGRNE - Product Insert

HTRF Product Catalog

All your HTRF assays in one document! - Catalog

A guide to Homogeneous Time Resolved Fluorescence

General principles of HTRF - Guides

How HTRF compares to Western Blot and ELISA

Get the brochure about technology comparison. - Brochures

Webinar | Association Rate Constant: The Unsung Hero of Drug Discovery Kinetics

Available On-demand - Videos

Easy method for kinetic binding determination

How to revolutionize your kinetic binding demonstration with HTRF kinase binding platform assays - Application Notes

GPCRs Pharmacology

A comprehensive overview of pharmacology's main ligands - Guides

Safety Data Sheet Halo-Green Subst / SHALOGRNE

SHALOGRNE - Safety Data Sheet

Plate Reader Requirement

Choosing the right microplate reader ensures you’ll get an optimal readout. Discover our high performance reader, or verify if your lab equipment is going to be compatible with this detection technology.

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