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Halo tag A2A AdenosineR cloned plasmid HTRF®

The Tag-lite A2A Adenosine plasmid is used to transiently or stably transfect cells for the purpose of developing a A2A Adenosine receptor binding assay.
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The Tag-lite A2A Adenosine plasmid is used to transiently or stably transfect cells for the purpose of developing a A2A Adenosine receptor binding assay.
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Overview

Over the past few years, SNAP/HALO-Tag technology combined with TR-FRET has paved the way for the development of many non-radioactive, no-wash, binding assays.

The method is based on transfecting cells using plasmids encoding a SNAP- or HALO-Tag and subsequently labeling them with Terbium. Cisbio offers a large collection of such plasmids. All GPCR genes are cloned in an expression vector directly downstream from a CMV promoter, and are provided ready for protein expression and labeling.

All information on this page pertains to the Tag-lite plasmid cloned with the A2A Adenosine receptor.

Benefits

  • FULL LENGTH RECEPTOR
  • HIGH EXPRESSION SYSTEM
  • VALIDATED IN EXPRESSION

Evaluation of a Tag-lite binding assay for a class B receptor

In collaboration with Boehringer Ingelheim - Scientific Presentations

Ultra HTS at Bayer: use of IP-One and Tag-lite assays in GPCR drug discovery

In collaboration with Bayer - Scientific Presentations

Tag-lite binding assays

The easy way to approach biology without radioactivity - Flyers

Understanding GPCRs is the key to improved DD

In collaboration with GEN - Guides

Tag-lite, the easiest solution for studying GPCR-ligands interactions

The gold standard technology for receptor binding studies - Videos

Transient Transfection Procedure

Cell transfection protocol - Product Insert

Labeling procedure Tag-lite

Cell surface receptor labeling - Product Insert

HTRF Product Catalog 2020 July update

All your HTRF assays in one document! - Catalog

A guide to Homogeneous Time Resolved Fluorescence

General principles of HTRF - Guides

Kinetic adenosine receptor family binding assays

Case study about kinetic binding characterization provided by the combination of Cisbio and Excellerate bioscience expertise. - Application Notes

How HTRF compares to Western Blot and ELISA

Get the brochure about technology comparison. - Brochures

Easy method for kinetic binding determination

How to revolutionize your kinetic binding demonstration with HTRF kinase binding platform assays - Application Notes

GPCRs Pharmacology

A comprehensive overview of pharmacology's main ligands - Guides

Safety Data Sheet pHalo-A2A-R / PHTA2A

PHTA2A - Safety Data Sheet

Plate Reader Requirement

Choosing the right microplate reader ensures you’ll get an optimal readout. Discover our high performance reader, or verify if your lab equipment is going to be compatible with this detection technology.

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