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Free and bound Insulin immunoradiometric assay kit

Free and bound Insulin immunoradiometric assay kit RIA

The BI-INS-IRMA kit is an immunoradiometric assay used on serum or EDTA or heparined plasma samples for a sensitive and specific measure of human insulin.

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The BI-INS-IRMA kit is an immunoradiometric assay used on serum or EDTA or heparined plasma samples for a sensitive and specific measure of human insulin.

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Overview

Diagnosis diabetes mellitus

Detect diabetes mellitus before clinical onset

Monitor metabolic control in diabetic patients

Use as prognosis factor for the risk of coronary incident

Diagnose insulinoma

Benefits

HIGH SENSITIVITY
NO CROSS-REACTION WITH PROINSULIN

Assay principle: sandwich

Two anti-insulin monoclonal antibodies are used to form a sandwich. The first monoclonal antibody is adsorbed onto the tube walls, while the second is radiolabelled with iodine-125.

Two alternative assay procedures:

1- measurement of immunoreactive insulin (free insulin + insulin bound to anti-insulin antibodies): 2h at RT / shaking

2- measurement of free insulin: 15 min centrifugation + 2h at RT / shaking