Development of an HTRF assay which opens up the possibility of extending...
Europium cryptate-NH2 labeling reagent HTRF®
Europium cryptate-NH2 labeling reagent enables Europium cryptate labeling of biomolecules.
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Ease-of-use
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Long stability
Overview
Europium cryptate-NH2, is a do-it-yourself solution for labeling peptides, oligonucleotides or proteins with Europium cryptate.This reagent is ideal for experienced users familiar with labeling and chromatography. Used in combination with bi-functional reagents such as carbodiimides and SMCC/SPDP. Maleimide group generated by SMCC activation of the Europium cryptate-NH2 reacts with the thiol generated by SPDP/DTT treatment on the biomolecules to label.
Benefits
- SPDP/SMCC COUPLING
- CUSTOM TR-FRET ASSAY SETUP
- FAST LABELING PROCEDURE
Assay protocol
As shown here, preactivation of both the Europium cryptate and the biomolecule may be required. Recovery of labeled material can be achieved using conventional chromatographic methods, such as gel filtration or HPLC. The purification devices required for recovering labeled material are not delivered with these reagents.
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Safety Data Sheet Eu-TBP-NH2 / 65EU2ABA
65EU2ABA - Safety Data Sheet
Safety Data Sheet (DEU) Eu-TBP-NH2 / 65EU2ABA
65EU2ABA - Safety Data Sheet
Safety Data Sheet (ELL) Eu-TBP-NH2 / 65EU2ABA
65EU2ABA - Safety Data Sheet
Safety Data Sheet (FRA-FR) Eu-TBP-NH2 / 65EU2ABA
65EU2ABA - Safety Data Sheet
Safety Data Sheet (ITA) Eu-TBP-NH2 / 65EU2ABA
65EU2ABA - Safety Data Sheet
Safety Data Sheet (SPA) Eu-TBP-NH2 / 65EU2ABA
65EU2ABA - Safety Data Sheet
Safety Data Sheet (ENG-GB) Eu-TBP-NH2 / 65EU2ABA
65EU2ABA - Safety Data Sheet
Safety Data Sheet (ENG-US) Eu-TBP-NH2 / 65EU2ABA
65EU2ABA - Safety Data Sheet
Safety Data Sheet Eu-TBP-NH2 / 65EU2ABB
65EU2ABB - Safety Data Sheet
Safety Data Sheet (DEU) Eu-TBP-NH2 / 65EU2ABB
65EU2ABB - Safety Data Sheet
Safety Data Sheet (ELL) Eu-TBP-NH2 / 65EU2ABB
65EU2ABB - Safety Data Sheet
Safety Data Sheet (FRA-FR) Eu-TBP-NH2 / 65EU2ABB
65EU2ABB - Safety Data Sheet
Safety Data Sheet (ITA) Eu-TBP-NH2 / 65EU2ABB
65EU2ABB - Safety Data Sheet
Safety Data Sheet (SPA) Eu-TBP-NH2 / 65EU2ABB
65EU2ABB - Safety Data Sheet
Safety Data Sheet (ENG-GB) Eu-TBP-NH2 / 65EU2ABB
65EU2ABB - Safety Data Sheet
Safety Data Sheet (ENG-US) Eu-TBP-NH2 / 65EU2ABB
65EU2ABB - Safety Data Sheet
Plate Reader Requirement
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