Total H3 cellular control lysate
EPIgeneous H3K36Me2 cellular kit HTRF®
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Cell-based
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High sensitivity
Overview
This cellular assay has been developed with optimized reagents and protocols for the direct detection of endogenous levels of H3K36Me2. This HTRF® format uses a very specific antibody pair combined with a highly efficient nucleus extraction which enables the assay to be used with a variety of cell backgrounds, and for the study of important methyltransferases and demethylases targets, such as JMJD2A. The H3K36Me2 assay can be used for adherent or suspension cells, primary or secondary screening and inhibitor studies.
Benefits
- COMPOUND SCREENING AND OPTIMIZATION
- ENDOGENOUS CELL EXPRESSION
- CLOSER TO PHYSIOLOGY
Assay principle
Two-plate assay protocol
One-plate assay protocol
HTRF detection of H3K36Me2 with two plate protocol on HeLa cells.
Detection of H3K36Me2 with 2 different cell lines
HTRF detection of H3K36Me2 mark with the two plate protocol in two cell lines: HeLa and U2OS. Cells were seeded at various densities in a 96-well plate and incubated for 24h before lysis without medium. Detection of H3K36Me2 was performed in a 384w white plate on a BMG Pherastar FS Flash lamp reader. For both cell lines, a hook effect was observed for the 30,000 cells per well concentration. For the same cellular concentration, U2OS showed an increase level of dimethylation on H3K36.
Antibody Specificity
The Tools of Tomorrow,when you need them Today
EPIgeneous cellular assays for measuring epigenetic modifications - Flyers
EPIgeneous Cell Based Assay Lysis Buffer Guide
EPIgeneous Cell Based Assay Lysis Buffer Guide - Technical Notes
Product Insert Cell H3K36Me2 Kit / 62KD2PAD
62KD2PAD - Product Insert
Product Insert Cell H3K36Me2 Kit / 62KD2PAE
62KD2PAE - Product Insert
HTRF Product Catalog 2020 July update
All your HTRF assays in one document! - Catalog
A guide to Homogeneous Time Resolved Fluorescence
General principles of HTRF - Guides
How HTRF compares to Western Blot and ELISA
Get the brochure about technology comparison. - Brochures
Safety Data Sheet (DEU) Cell H3K36Me2 Kit / 62KD2PAD
62KD2PAD - Safety Data Sheet
Safety Data Sheet (ELL) Cell H3K36Me2 Kit / 62KD2PAD
62KD2PAD - Safety Data Sheet
Safety Data Sheet (FRA-FR) Cell H3K36Me2 Kit / 62KD2PAD
62KD2PAD - Safety Data Sheet
Safety Data Sheet (ITA) Cell H3K36Me2 Kit / 62KD2PAD
62KD2PAD - Safety Data Sheet
Safety Data Sheet (SPA) Cell H3K36Me2 Kit / 62KD2PAD
62KD2PAD - Safety Data Sheet
Safety Data Sheet (ENG-GB) Cell H3K36Me2 Kit / 62KD2PAD
62KD2PAD - Safety Data Sheet
Safety Data Sheet (ENG-US) Cell H3K36Me2 Kit / 62KD2PAD
62KD2PAD - Safety Data Sheet
Safety Data Sheet (DEU) Cell H3K36Me2 Kit / 62KD2PAE
62KD2PAE - Safety Data Sheet
Safety Data Sheet (ELL) Cell H3K36Me2 Kit / 62KD2PAE
62KD2PAE - Safety Data Sheet
Safety Data Sheet (FRA-FR) Cell H3K36Me2 Kit / 62KD2PAE
62KD2PAE - Safety Data Sheet
Safety Data Sheet (ITA) Cell H3K36Me2 Kit / 62KD2PAE
62KD2PAE - Safety Data Sheet
Safety Data Sheet (SPA) Cell H3K36Me2 Kit / 62KD2PAE
62KD2PAE - Safety Data Sheet
Safety Data Sheet (ENG-GB) Cell H3K36Me2 Kit / 62KD2PAE
62KD2PAE - Safety Data Sheet
Safety Data Sheet (ENG-US) Cell H3K36Me2 Kit / 62KD2PAE
62KD2PAE - Safety Data Sheet
Plate Reader Requirement
Choosing the right microplate reader ensures you’ll get an optimal readout. Discover our high performance reader, or verify if your lab equipment is going to be compatible with this detection technology.
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