CXCR4R stably expressing cells
Tag-lite CXCR4 stably expressing cells
CXCR4R fluorescent probe HTRF®
This CXCR4 receptor red agonist is a SDF1alpha derivative labeled with a red emitting HTRF fluorescent probe.
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Non-radioactive
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No-wash
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Cell-based
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Equilibrium & Kinetic binding compatible
Overview
Tag-lite receptor binding assays use either a green or a red fluorescent ligand to function.
This CXCR4 receptor red agonist is a SDF1 alpha derivative labeled with a red emitting HTRF fluorescent probe.
This ligand is best suited for use in the Chemokine CXCR4 receptor binding assay.
Benefits
- FLUORESCENTLY LABELED LIGAND
- HIGHLY STABLE
- VALIDATED IN BINDING
Assay principle
Running a receptor binding assay using Tag-lite is as easy as it can get. Simply dispense 10 µL of labeled cells into each well, followed by 5 µL of labeled ligand and 5 µL of the compound you wish to test. Like all HTRF assays, Tag-lite assays do not require any washing steps. A diagram of the procedure to be followed is given on the right.
Saturation binding (KD)
A saturation binding assay measures total and non-specific binding for increasing concentrations of ligand under equilibrium conditions. To perform the assay, the fluorescent ligand is titrated into a solution containing a fixed amount of labeled cells and then incubated to equilibrium. The HTRF ratio obtained from this titration is the total binding.
Watch this video explaining how to run a saturation binding assay using Tag-lite.
Competitive binding (KI)
A competitive binding assay is performed to measure the dissociation constant, Ki. To perform the assay, the compound is titrated into a solution containing a fixed concentration of fluorescent ligand and a fixed amount of cells.
Watch this video explaining how to run a competitive binding assay using Tag-lite.
HTRF Ligand Binding Assay for the Chemokine Receptor CXCR4
Tag-lite assay read on Synergy (BioTek) - Application Notes
Evaluation of a Tag-lite binding assay for a class B receptor
In collaboration with Boehringer Ingelheim - Scientific Presentations
Ultra HTS at Bayer: use of IP-One and Tag-lite assays in GPCR drug discovery
In collaboration with Bayer - Scientific Presentations
The easy way to approach biology without radioactivity - Flyers
In collaboration with BioTek - Posters
Challenge the limits of binding kinetics studies - Application Notes
Understanding GPCRs is the key to improved DD
In collaboration with GEN - Guides
Webinar: The kinetics of drug-receptor binding: why it is important and how we can measure it
Featuring a panel of experts - Videos
Tag-lite, the easiest solution for studying GPCR-ligands interactions
The gold standard technology for receptor binding studies - Videos
Product Insert CXCR4-R red ago / L0012RED
L0012RED - Product Insert
HTRF Product Catalog 2020 July update
All your HTRF assays in one document! - Catalog
A guide to Homogeneous Time Resolved Fluorescence
General principles of HTRF - Guides
How HTRF compares to Western Blot and ELISA
Get the brochure about technology comparison. - Brochures
Webinar | Association Rate Constant: The Unsung Hero of Drug Discovery Kinetics
Available On-demand - Videos
Easy method for kinetic binding determination
How to revolutionize your kinetic binding demonstration with HTRF kinase binding platform assays - Application Notes
A comprehensive overview of pharmacology's main ligands - Guides
Safety Data Sheet CXCR4-R red ago / L0012RED
L0012RED - Safety Data Sheet
Safety Data Sheet (DEU) CXCR4-R red ago / L0012RED
L0012RED - Safety Data Sheet
Safety Data Sheet (ELL) CXCR4-R red ago / L0012RED
L0012RED - Safety Data Sheet
Safety Data Sheet (FRA-FR) CXCR4-R red ago / L0012RED
L0012RED - Safety Data Sheet
Safety Data Sheet (ITA) CXCR4-R red ago / L0012RED
L0012RED - Safety Data Sheet
Safety Data Sheet (SPA) CXCR4-R red ago / L0012RED
L0012RED - Safety Data Sheet
Safety Data Sheet (ENG-GB) CXCR4-R red ago / L0012RED
L0012RED - Safety Data Sheet
Safety Data Sheet (ENG-US) CXCR4-R red ago / L0012RED
L0012RED - Safety Data Sheet
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