Cortisol in human serum and saliva radioimmunoassay kit RIA

The CORT-CT2 kit is a radioimmunoassay that measures seric and salivary cortisol levels for the examination of the hypothalamic-pituitary adrenal cortex axis.

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The CORT-CT2 kit is a radioimmunoassay that measures seric and salivary cortisol levels for the examination of the hypothalamic-pituitary adrenal cortex axis.

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Overview

Explore the hypothalamic-pituitary adrenal cortex axis

Use an essential screening tool in hypocortisolism and hypercortisolism

Determine cortisol levels after stimulation and suppression tests

Discriminate between primary and secondary adrenal insufficiency Differentially diagnose Cushing’s syndromes

Benefits

ACCURATE AND RELIABLE ASSAY
STANDARDIZED AGAINST MASS SPECTROMETRY

Assay principle: competition

The principle of the assay is based on a competition between iodine-125 radiolabeled cortisol and cortisol contained in the samples for a fixed and limited number of anti-cortisol polyclonal antibody binding sites adsorbed onto the tube walls.

One-step assay procedure: 1- Serum: 2h at 37°C 2- Saliva: 30 min at 37°C

Features

Working range: sérum: 20 – 2000 nmol/L

Working range:salive:1 – 100 nmol/L

Sample: serum: 20 µl;saliva: 150 µL

Analytical sensitivity: sérum: 6.0 nmol/L serum;saliva: 3.0 nmol/L

Normal values

Healthy subjects (20 59 years)	Serum (nmol/L)		Saliva (nmol/L)
Healthy subjects (20 59 years)	8 10 am	3 5 pm	6 8 am	6 8 pm
n	220	60	126	124
Means (nmol/L)	308	182	27.9	6.7
Reference interval (nmol/L)	131 - 642	61 - 429	10.4 - 45.9	3.2 - 14.7
Confidence intervals 0.90
For lower reference limit	104 - 146		6.5 - 12.1	2.6 - 3.5
For upper reference limit	576 - 709		45.4 - 52.9	12.9 - 16.2