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Beta2 adrenergicR fluorescent probe HTRF®

This Beta adrenergic receptor green antagonist is a (+/-) propranolol derivative labeled with a green emitting HTRF fluorescent probe.
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  • Non-radioactive Non-radioactive
  • No-wash No-wash
  • Cell-based Cell-based
  • Equilibrium & Kinetic binding compatible Equilibrium & Kinetic binding compatible
This Beta adrenergic receptor green antagonist is a (+/-) propranolol derivative labeled with a green emitting HTRF fluorescent probe.
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Overview

Tag-lite receptor binding assays use either a green or a red fluorescent ligand to function.

This Beta adrenergic receptor green antagonist is a (+/-) propranolol derivative labeled with a green emitting HTRF fluorescent probe.

This ligand is best suited for use in the Adrenoceptor Beta2 receptor binding assay.

Benefits

  • FLUORESCENTLY LABELED LIGAND
  • HIGHLY STABLE
  • VALIDATED IN BINDING

Assay principle

Running a receptor binding assay using Tag-lite is as easy as it can get. Simply dispense 10 µL of labeled cells into each well, followed by 5 µL of labeled ligand and 5 µL of the compound you wish to test. Like all HTRF assays, Tag-lite assays do not require any washing steps. A diagram of the procedure to be followed is given on the right.
Diagram of a receptor binding assay using the Tag-lite protocol

Saturation binding (KD)

A saturation binding assay measures total and non-specific binding for increasing concentrations of ligand under equilibrium conditions. To perform the assay, the fluorescent ligand is titrated into a solution containing a fixed amount of labeled cells and then incubated to equilibrium. The HTRF ratio obtained from this titration is the total binding.
Diagram of a saturation binding assay using Tag-lite
Representative data obtained when running a saturation binding assay

Watch this video explaining how to run a saturation binding assay using Tag-lite.

Competitive binding (KI)

A competitive binding assay is performed to measure the dissociation constant, Ki. To perform the assay, the compound is titrated into a solution containing a fixed concentration of fluorescent ligand and a fixed amount of cells.
Diagram of a competitive binding assay using Tag-lite
Representative data obtained when running a competitive binding assay

Watch this video explaining how to run a competitive binding assay using Tag-lite.

Evaluation of a Tag-lite binding assay for a class B receptor

In collaboration with Boehringer Ingelheim - Scientific Presentations

Ultra HTS at Bayer: use of IP-One and Tag-lite assays in GPCR drug discovery

In collaboration with Bayer - Scientific Presentations

Tag-lite binding assays

The easy way to approach biology without radioactivity - Flyers

Kinetics of the ß2 Adrenergic receptor binding and signaling

A look at the kinetics of cAMP production in cells - Application Notes

Understanding GPCRs is the key to improved DD

In collaboration with GEN - Guides

Tag-lite, the easiest solution for studying GPCR-ligands interactions

The gold standard technology for receptor binding studies - Videos

HTRF Product Catalog 2020 July update

All your HTRF assays in one document! - Catalog

A guide to Homogeneous Time Resolved Fluorescence

General principles of HTRF - Guides

How HTRF compares to Western Blot and ELISA

Get the brochure about technology comparison. - Brochures

Easy method for kinetic binding determination

How to revolutionize your kinetic binding demonstration with HTRF kinase binding platform assays - Application Notes

GPCRs Pharmacology

A comprehensive overview of pharmacology's main ligands - Guides

Plate Reader Requirement

Choosing the right microplate reader ensures you’ll get an optimal readout. Discover our high performance reader, or verify if your lab equipment is going to be compatible with this detection technology.

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