Aquaporin-4 autoantibody (AQP4Ab) ELISA kit ELISA
Diagnosis neuromyelitis optica (NMO / Devic’s syndrome) or neuromyelitis optica spectrum disorder (NMOSD)
Distinguish NMOSD from multiple sclerosis (MS) at an early stage of the disease
- ACCURATE AND RELIABLE ENZYMATIC ASSAY
AQP4 molecules are coated onto the ELISA plate wells. AQP4-biotin complexes constitute the liquid phase. AQP4 Ab from the sample will bind to the AQP4 coated on the well and interact with AQP4-biotin to form a bridge. The bound AQP4-biotin is detected through the addition of streptavidin peroxidase (SA-POD). The unbound SA-POD in excess is washed off before addition of the peroxidase substrate TMB.
Assay procedure: 2h at RT / shaking + 20 min at RT / shaking + 20 min at RT in the dark.
Working range: 1.5 – 80 U/mL
Sample: serum – 50 µL
Analytical sensitivity: 0.17 U/mL