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Amyloid beta1-40 Kit HTRF®

The Amyloid ß1-40 kit is designed for the simple and rapid quantification of the peptide in cell-based formats.
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  • Ready-to-use Ready-to-use
  • Highly accurate Highly accurate
  • Faster and more convenient than ELISA Faster and more convenient than ELISA
The Amyloid ß1-40 kit is designed for the simple and rapid quantification of the peptide in cell-based formats.
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Overview

Amyloid ß1-40 and ß1-42 peptides are involved in the appearance of Alzheimer's disease symptoms. The production of these two amyloid peptides is regulated by the activity of 2 enzymes, ß and γ secretase, which cleave the amyloid precursor protein. This kit is intended to measure amyloid ß1-40 peptides in cell supernatant or in whole cells. This assay is a fast alternative to ELISA, thanks to the ease-of-use of our Add and Read method.

Benefits

  • HIGHLY SPECIFIC
  • READY-TO-USE KIT

Assay principle

The HTRF Amyloid ß1-40 assay is a sandwich immunoassay using two monoclonal antibodies that recognize different epitopes within the ß1-40 peptide. One antibody is labelled with Eu3+-Cryptate and the other with XL-665. The assay can be used with whole cells or cell supernatant.
Amyloid ß1-40 assay principle

Assay Protocol

The Amyloid ß1-40 assay features a streamlined protocol with only two incubation steps:

- Cell stimulation by target compounds

- Amyloid ß1-40 detection using HTRF reagents

The antibodies labelled with HTRF donor and acceptor may be pre-mixed and added in a single dispensing step to further speed up the assay procedure. This protocol requires a single 24-hour incubation at 4°C following cell stimulation.

Amyloid ß1-40 assay protocol

Key features

Detection limit: 13.64 pg/mL
Dynamic range: 25 to 1600 pg/mL
Sample compatibility: Cell-based or cell supernatants
Specificity: No cross-reaction with Amyloid ß 1-42 and ß 1-43
Amyloid ß1-40 standard curve

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Plate Reader Requirement

Choosing the right microplate reader ensures you’ll get an optimal readout. Discover our high performance reader, or verify if your lab equipment is going to be compatible with this detection technology.

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