Alpha-SMA quantification in cell lysates
Amyloid ß1-40 and ß1-42 peptides are involved in the appearance of Alzheimer's disease symptoms. The production of these two amyloid peptides is regulated by the activity of 2 enzymes, ß and γ secretase, which cleave the amyloid precursor protein. This kit is intended to measure amyloid ß1-40 peptides in cell supernatant or in whole cells. This assay is a fast alternative to ELISA, thanks to the ease-of-use of our Add and Read method.
The Amyloid ß1-40 assay features a streamlined protocol with only two incubation steps:
- Cell stimulation by target compounds
- Amyloid ß1-40 detection using HTRF reagents
The antibodies labelled with HTRF donor and acceptor may be pre-mixed and added in a single dispensing step to further speed up the assay procedure. This protocol requires a single 24-hour incubation at 4°C following cell stimulation.
|Detection limit:||13.64 pg/mL|
|Dynamic range:||25 to 1600 pg/mL|
|Sample compatibility:||Cell-based or cell supernatants|
|Specificity:||No cross-reaction with Amyloid ß 1-42 and ß 1-43|
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